Single-molecule surface enhanced resonance Raman spectroscopy of the enhanced green fluorescent protein

被引：135

作者：

Habuchi, S

Cotlet, M

Gronheid, R

Dirix, G

Michiels, J

Vanderleyden, J

De Schryver, FC

Hofkens, J

机构：

[1] Katholieke Univ Leuven, Dept Chem, B-3001 Heverlee, Belgium

[2] Ctr Microbial & Plant Genet, B-3001 Heverlee, Belgium

[3] Catholic Univ Louvain, Unite CMAT, B-1348 Louvain, Belgium

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 28期

关键词：

D O I：

10.1021/ja0353311

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the present contribution, we demonstrated that surface-enhanced resonance Raman scattering spectra from single green fluorescent proteins (GFPs) were obtained. The most important findings are the direct detection of the conversion between a deprotonated and a protonated form of the chromophore at the single-molecule level via the corresponding vibrational fingerprints, and the fact that the enhanced green fluorescent protein (EGFP) also shows a high surface enhanced resonance Raman scattering (SERRS) signal. Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level. Copyright © 2003 American Chemical Society.

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页码：8446 / 8447

页数：2

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