Distinguishing Acute from Chronic Hepatitis C Virus (HCV) Infection Based on Antibody Reactivities to Specific HCV Structural and Nonstructural Proteins

被引：30

作者：

Araujo, Aufra C. ^{[1
]}

Astrakhantseva, Irina V. ^{[1
,2
]}

Fields, Howard A. ^{[1
]}

Kamili, Saleem ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div Viral Hepatitis, Branch Lab, Atlanta, GA 30333 USA

[2] RPC Diagnost Syst, Nizhnii Novgorod, Russia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 01期

关键词：

DIAGNOSIS; ANTIGEN;

D O I：

10.1128/JCM.01064-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Currently available serological assays for detection of antibodies to hepatitis C virus (HCV) cannot reliably discriminate acute from chronic HCV infection. We developed a multiplexed, flow-cytometric microsphere immunoassay to measure anti-HCV-IgG reactivities to the core, NS3, NS4, and NS5 HCV recombinant proteins and applied it to 99 serum samples from 24 anti-HCV seroconverters and 141 anti-HCV-IgG and HCV RNA-positive plasma specimens from chronically infected people. Differences in the geometric means or means of signal/cutoff ratios between the two sample sets were statistically significant for all the antigens tested. A multivariate logistic regression model correctly classified the samples in two groups, with a cross-validation accuracy of 90.8% for the acute group and 97.2% for the chronic group. The immunoassay described has the potential to distinguish acute from chronic HCV infection.

引用

页码：54 / 57

页数：4

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