Studies on the extraction and purification of phytic acid from rice bran

被引:80
作者
Canan, Cristiane [1 ]
Lisboa Cruz, Felipe Tsuruta [1 ]
Delaroza, Fernanda [1 ]
Casagrande, Rubia [2 ]
Mendes Sarmento, Cleonice Pereira [3 ]
Shimokomaki, Massami [1 ]
Ida, Elza Iouko [1 ]
机构
[1] Univ Estadual Londrina, Dept Food Sci & Technol, Londrina, Parana, Brazil
[2] Univ Estadual Londrina, Dept Pharmaceut Sci, Londrina, Parana, Brazil
[3] Fed Technol Univ Parana, Dept Food Technol, Medianeira, Parana, Brazil
关键词
Myo-inositol hexaphosphate; Cereals; Response surface methodology; Analytical process; Antioxidants; Food analysis; Food composition; CORN GERM; PHYTATE; ANTIOXIDANT; STABILITY; CULTIVAR; BINDING; MODEL; MEAT;
D O I
10.1016/j.jfca.2010.12.014
中图分类号
O69 [应用化学];
学科分类号
070301 [无机化学];
摘要
The objective of this work was to develop rice bran IRGA 417 phytic acid (PA) extraction and purification techniques. For the extraction of PA, a complete 2(4) factorial design with triplicates at the central point was used, and the effects of concentration of rice bran and HCI, time and temperature were investigated. During purification, different pH values were tested with addition of 1.5 M Na2CO3 or 4.0 M NaOH. The results obtained by the statistical analysis of the factorial design showed that temperature, time and HCI concentration influenced the PA extraction technique significantly (p <= 0.05), whereas the concentration of rice bran had no influence. The content of PA was evaluated in all the stages of purification and it was possible to establish an improved methodology of extraction and purification with high purity and yields. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:1057 / 1063
页数:7
相关论文
共 45 条
[1]
Quantitative analysis of phytate globoids isolated from wheat bran and characterization of their sequential dephosphorylation by wheat phytase [J].
Bohn, Lisbeth ;
Josefsen, Lone ;
Meyer, Anne S. ;
Rasmussen, Soren K. .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (18) :7547-7552
[2]
IMPROVED DETERMINATION OF PHYTATE BY ION-EXCHANGE CHROMATOGRAPHY [J].
BOS, KD ;
VERBEEK, C ;
VANEEDEN, CHP ;
SLUMP, P ;
WOLTERS, MGE .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1991, 39 (10) :1770-1772
[3]
In vitro evaluation of quercetin cutaneous absorption from topical formulations and its functional stability by antioxidant activity [J].
Casagrande, Rubia ;
Georgetti, Sandra R. ;
Verri, Waldiceu A., Jr. ;
Borin, Maria F. ;
Lopez, Renata F. V. ;
Fonseca, Maria J. V. .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2007, 328 (02) :183-190
[4]
MICRODETERMINATION OF PHOSPHORUS [J].
CHEN, PS ;
TORIBARA, TY ;
WARNER, H .
ANALYTICAL CHEMISTRY, 1956, 28 (11) :1756-1758
[5]
Determination of metals in bottled coconut water using an inductively coupled plasma optical emission spectrometer [J].
de Sousa, RA ;
Silva, JCJ ;
Baccan, N ;
Cadore, S .
JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2005, 18 (05) :399-408
[6]
Dean J.A., 1960, FLAME PHOTOMETRY
[7]
ELLIS R, 1986, CEREAL CHEM, V63, P58
[8]
CORN GERM PHYTIC ACID ANTIOXIDANT ACTIVITY EVALUATION. [J].
Filgueiras, Cristina Tostes ;
Soares, Adriana Lourenco ;
Shimokomaki, Massami ;
Ida, Elza Iouko ;
Casagrande, Rubia .
QUIMICA NOVA, 2009, 32 (07) :1787-1791
[9]
Fuh WS, 2001, J SCI FOOD AGR, V81, P1419, DOI 10.1002/jsfa.962
[10]
ANTIOXIDANT FUNCTIONS OF PHYTIC ACID [J].
GRAF, E ;
EATON, JW .
FREE RADICAL BIOLOGY AND MEDICINE, 1990, 8 (01) :61-69