Protective effect of the apoptosis-sensing nanoparticle AnxCLIO-Cy5.5

被引：7

作者：

Chen, Howard H. ^{[2
]}

Feng, Yan ^{[3
]}

Zhang, Ming ^{[3
]}

Chao, Wei ^{[3
]}

Josephson, Lee ^{[2
,5
]}

Shaw, Stanley Y. ^{[4
]}

Sosnovik, David E. ^{[1
,2
]}

机构：

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Mol Imaging Res,CNY, Charlestown, MA 02129 USA

[2] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Martinos Ctr Biomed Imaging, Charlestown, MA 02129 USA

[3] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Anesthesia & Crit Care, Charlestown, MA 02129 USA

[4] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Ctr Syst Biol, Boston, MA USA

[5] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Translat Nucl Med & Mol Imaging, Charlestown, MA 02129 USA

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2012年 / 8卷 / 03期

关键词：

Apoptosis; Magnetic nanoparticle; Cardioprotection; Iron oxide; Gene expression; ACUTE MYOCARDIAL-INFARCTION; IRON-OXIDE NANOPARTICLES; ANNEXIN A5; CELL-DEATH; NONINVASIVE DETECTION; REPERFUSION INJURY; IN-VIVO; HEART; PHOSPHATIDYLSERINE; PARTICLES;

D O I：

10.1016/j.nano.2011.06.012

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

The diagnostic utility of the apoptosis-sensing nanoparticle (NP), AnxCLIO-Cy5.5, is well established. Here we sought to define the pathophysiological impact of the nanoparticle (NP) on apoptotic cells. Confocal microscopy showed that AnxCLIO-Cy5.5 remained bound to apoptotic cell membranes for 3 hours but by 7 hours had become completely internalized. AnxCLIO-Cy5.5 exposure did not impact energetics, metabolism or caspase-3 activity in apoptotic cells. Gene expression in cells exposed to AnxCLIO-Cy5.5 did not reveal upregulation of pro-inflammatory or cell-death pathways. Moreover, exposure to AnxCLIO-Cy5.5 decreased the frequency of membrane rupture of early apoptotic cells. Similarly, in mice exposed to 1 hour of ischemia -reperfusion, the injection of AnxCLIO-Cy5.5 at the onset of reperfusion reduced infarct size/area at risk by 16.2%. Our findings suggest that AnxCLIO-Cy5.5 may protect apoptotic cells by stabilizing their cell membranes and has the potential to become a theranostic agent, capable of both identifying and salvaging early apoptotic cells.

引用

页码：291 / 298

页数：8

共 27 条

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Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging [J].