Pyrene-Excimer Probes Based on the Hybridization Chain Reaction for the Detection of Nucleic Acids in Complex Biological Fluids

被引：730

作者：

Huang, Jin ^{[3
,4
,5
,6
]}

Wu, Yanrong ^{[5
,6
]}

Chen, Yan ^{[1
,2
,3
,4
,5
,6
]}

Zhu, Zhi ^{[5
,6
]}

Yang, Xiaohai ^{[3
,4
]}

Yang, Chaoyong James ^{[1
,2
]}

Wang, Kemin ^{[3
,4
]}

Tan, Weihong ^{[3
,4
,5
,6
]}

机构：

[1] Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Key Lab Chem Biol Fujian Prov, Key Lab Analyt Sci,Coll Chem & Chem Engn, Xiamen 361005, Peoples R China

[2] Xiamen Univ, Dept Biol Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China

[3] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Biol, Changsha 410082, Hunan, Peoples R China

[4] Hunan Univ, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

[5] Univ Florida, Genet Inst, Ctr Res Bio Nano Interface, Dept Chem, Gainesville, FL 32611 USA

[6] Univ Florida, Genet Inst, Shands Canc Ctr, Gainesville, FL 32611 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 02期

基金：

对外科技合作项目（国际科技项目）;

关键词：

amplification; DNA; excimers; fluorescent probes; hybridization chain reaction; MOLECULAR BEACONS; DNA; AMPLIFICATION;

D O I：

10.1002/anie.201005375

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Make it a double: Complementary probes with two pyrene labels were engineered for the amplification of a target DNA sequence. In the stem-closed conformation of the probes in the absence of the target, the two pyrene moieties were separated. The target propagated hybridization chain reactions to bring pyrene moieties on neighboring probes close together to form fluorescent pyrene excimers (see picture). © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：401 / 404

页数：4

共 21 条

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GENETIC-DISEASE DETECTION AND DNA AMPLIFICATION USING CLONED THERMOSTABLE LIGASE [J].