Genetic characterization of Puumala hantavirus strains from Belgium: evidence for a distinct phylogenetic lineage

被引：21

作者：

Escutenaire, S

Chalon, P

Heyman, P

Van der Auwera, G

van der Groen, G

Verhagen, R

Thomas, I

Karelle-Bui, L

Vaheri, A

Pastoret, PP

Plyusnin, A

机构：

[1] Univ Liege, Fac Med Vet, Dept Immunol Vaccinol, B-4000 Liege, Belgium

[2] Queen Astrid Mil Hosp, Res Lab Vector Borne Dis, B-1120 Brussels, Belgium

[3] Inst Trop Med, Dept Microbiol, B-2000 Antwerp, Belgium

[4] RUCA, Dept Evolutionary Biol, B-2020 Antwerp, Belgium

[5] Louis Pasteur Publ Hlth Inst, Dept Virol, B-1050 Brussels, Belgium

[6] Univ Helsinki, Haartman Inst, Dept Virol, FIN-00014 Helsinki, Finland

来源：

VIRUS RESEARCH | 2001年 / 74卷 / 1-2期

关键词：

hantavirus; puumala virus; genetics; Belgium;

D O I：

10.1016/S0168-1702(00)00224-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Puumala hantavirus (PUUV) sequences were recovered from red bank voles (Clethrionomys glareolus) trapped between 1996 and 1998 in four localities of southern Belgium: Thuin: Montbliart, Momignies and Couvin. In addition, three PUUV isolates originating from bank voles trapped in the 1980s in southern (Montbliart) and northern (Turnhout) Belgium were genetically characterized. Analysis of the complete S and partial M segment sequences showed that the Belgian PUUV strains constitute a genetic lineage, distinct from other known PUUV lineages from Europe and Japan. This lineage also includes a wild strain (Cg-Erft) originating from a neighbouring area of Germany. Within the Belgian lineage, geographical clustering of genetic variants was observed. In the Montbliart site, the range of diversity between the most temporally distant strains (from 1986 and 1996-1998) was higher than between those from 1996 and 1998, suggesting slight genetic drift via accumulation of neutral or quasi-neutral substitutions with time. (C) 2001 Published by Elsevier Science B.V.

引用

页码：1 / 15

页数：15

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[31] Cell culture adaptation of Puumala hantavirus changes the infectivity for its natural reservoir, Clethrionomys glareolus, and leads to accumulation of mutants with altered genomic RNA S segment [J].