Stimulation of mitogen activated protein kinase by LDL and oxLDL in human U-937 macrophage-like cells

被引：53

作者：

Deigner, HP

Claus, R

机构：

[1] Pharmazeutisch-Chemisches Institut, Universität Heidelberg, 69120 Heidelberg

来源：

FEBS LETTERS | 1996年 / 385卷 / 03期

关键词：

mitogen activated protein kinase; lipoprotein; oxidized LDL; U937; cells;

D O I：

10.1016/0014-5793(96)00371-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitogen activated protein kinase in extracts of U-937 macrophage-like cells was stimulated by LDL and oxLDL. A maximum value (161% of the basal phosphotransferase activity) was obtained after 6 min exposure to oxidized LDL (27 mu g/ml) using APRTPGGRR peptide substrate. The activatory effect was more pronounced (LDL 181%, oxLDL 201%) when MAPK of stimulated cells was immunoprecipitated with anti-p42(MAPK) antibodies and phosphotransferase activity was assayed in immune complexes. Stimulation produced by oxLDL was inhibited by poly I, fucoidan, dextran sulfate and by the MAPKK inhibitor PD 098059 but not by PMA-mediated depletion of PKC or by pre-treatment with chloroquine or with pertussis toxin. These results suggest a direct mitogenic effect of LDL which, in the case of oxLDL, is dependent on scavenger receptor ligation but not on G-protein mediated or PKC-dependent signal transduction.

引用

页码：149 / 153

页数：5

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