Habenula "Cholinergic" Neurons Corelease Glutamate and Acetylcholine and Activate Postsynaptic Neurons via Distinct Transmission Modes

被引：273

作者：

Ren, Jing ^{[1
,2
]}

Qin, Chang ^{[1
,3
,4
]}

Hu, Fei ^{[1
,3
,4
]}

Tan, Jie ^{[1
]}

Qiu, Li ^{[5
]}

Zhao, Shengli ^{[5
]}

Feng, Guoping ^{[5
,6
,7
]}

Luo, Minmin ^{[1
,8
]}

机构：

[1] Natl Inst Biol Sci, Beijing 102206, Peoples R China

[2] Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China

[3] Chinese Acad Med Sci, Grad Program, Beijing 100730, Peoples R China

[4] Peking Union Med Coll, Beijing 100730, Peoples R China

[5] Duke Univ, Dept Neurobiol, Med Ctr, Durham, NC 27710 USA

[6] MIT, McGovern Inst Brain Res, Cambridge, MA 02139 USA

[7] MIT, Dept Brain & Cognit Sci, Cambridge, MA 02139 USA

[8] Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China

来源：

NEURON | 2011年 / 69卷 / 03期

关键词：

NICOTINIC RECEPTORS; RAT; RELEASE; SYSTEM; PATHWAYS;

D O I：

10.1016/j.neuron.2010.12.038

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

Acetylcholine is an important neurotransmitter, and the habenulo-interpeduncular projection is a major cholinergic pathway in the brain. To study the physiological properties of cholinergic transmission in the interpeduncular nucleus (IPN), we used a transgenic mouse line in which the light-gated cation channel ChannelRhodopsin-2 is selectively expressed in cholinergic neurons. Cholinergic axonal terminals were activated by light pulses, and postsynaptic responses were recorded from IPN neurons. Surprisingly, brief photostimulation produces fast excitatory. postsynaptic currents that are mediated by ionotropic glutamate receptors, suggesting wired transmission of glutamate. By contrast, tetanic photostimulation generates slow inward currents that are largely mediated by nicotinic acetylcholine receptors, suggesting volume transmission of acetylcholine. Finally, vesicular transporters for glutamate and acetylcholine are coexpressed on the same axonal terminals in the IPN. These results strongly suggest that adult brain "cholinergic" neurons can corelease glutamate and acetylcholine, but these two neurotransmitters activate postsynaptic neurons via different transmission modes.

引用

页码：445 / 452

页数：8

共 38 条

[1]

Simultaneous release of glutamate and acetylcholine from single magnocellular "cholinergic" basal forebrain neurons [J].