Cytoplasmic volume condensation is an integral part of mitosis

被引:85
作者
Habela, Christa W. [1 ]
Sontheimer, Harald [1 ]
机构
[1] Univ Alabama Birmingham, Ctr Glial Biol Med, Dept Neurobiol, Birmingham, AL 35294 USA
关键词
cancer; cell cycle; chloride channels; chromatin condensation; intracellular chloride; glioma; proliferation; volume regulation;
D O I
10.4161/cc.6.13.4357
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cell growth and osmotic volume regulation are undoubtedly linked to the progres sion of the cell cycle as with each division, a newly generated cell must compensate for loss of half of its volume to its sister cell. The extent to which size influences cell cycle decisions, however, is controversial in mammalian cells. Further, a mechanism by which cells can monitor and therefore regulate their size has not been fully elucidated. Despite an ongoing debate, there have been few studies which directly address the question in single cell real - time experiments. In this study we used fluorescent time - lapse imaging to quantitatively assess volume in individual spontaneously dividing cells throughout the cell cycle. Together with biophysical studies, these establish that the efflux of salt and water brings about a condensation of cytoplasmic volume as glioma cells progress through mitosis. As cells undergo this pre -mitotic condensation ( PMC) they approach a preferred cell volume preceding each division. This is functionally linked to chromatin condensa tion, suggesting that PMC plays an integral role in mitosis.
引用
收藏
页码:1613 / 1620
页数:8
相关论文
共 42 条
[31]   Checking out the G2/M transition [J].
Smits, VAJ ;
Medema, RH .
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2001, 1519 (1-2) :1-12
[32]  
Sveiczer A, 1996, J CELL SCI, V109, P2947
[33]   Expression of voltage-activated chloride currents in acute slices of human gliomas [J].
Ullrich, N ;
Bordey, A ;
Gillespie, GY ;
Sontheimer, H .
NEUROSCIENCE, 1998, 83 (04) :1161-1173
[34]   The elusive sizer [J].
Umen, JG .
CURRENT OPINION IN CELL BIOLOGY, 2005, 17 (04) :435-441
[35]   Chloride/anion channels in glial cell membranes [J].
Walz, W .
GLIA, 2002, 40 (01) :1-10
[36]  
WANG L, 2000, J PHYSL, V524, P35
[37]   Regulatory volume decrease is actively modulated during the cell cycle [J].
Wang, LW ;
Chen, LX ;
Zhu, LY ;
Rawle, M ;
Nie, SH ;
Zhang, J ;
Ping, Z ;
Cai, KR ;
Jacob, TJC .
JOURNAL OF CELLULAR PHYSIOLOGY, 2002, 193 (01) :110-119
[38]   Does size matter? [J].
Wells, WA .
JOURNAL OF CELL BIOLOGY, 2002, 158 (07) :1156-1159
[39]  
WONDERGEM R, 2001, J PHYSL, V532, P3
[40]  
YAMADA J, 2004, J PHYSL, V557, P3