Anti-inflammatory and immunomodulating properties of a sterol fraction from Sideritis foetens CLEM.

被引：115

作者：

Navarro, A ^{[1
]}

De las Heras, B ^{[1
]}

Villar, A ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Farm, Dept Farmacol, E-28040 Madrid, Spain

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2001年 / 24卷 / 05期

关键词：

sterols; Sideritis foetens; inflammation; polymorphonuclear leukocyte;

D O I：

10.1248/bpb.24.470

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A sterol fraction composed of campesterol (7.6%), stigmasterol (28.4%) and beta -sitosterol (61.1%) was obtained by activity-guided fractionation of the acetone extract of Sideritis foetens CLEM. This sterol fraction showed anti-inflammatory activity in irt vivo murine models of inflammation. It decreased carrageenan paw oedema in mice after oral administration of 30 and 60 mg/kg and inhibited mouse ear oedema induced by 12-O-tetradecanoylphorbol acetate (TPA) after topical application. Quantitation of the neutrophil specific marker myeloperoxidase (MPO) demonstrated that its topical anti-inflammatory activity was associated with reduction in neutrophil infiltration into inflamed tissues. Non-cytotoxic concentrations of the sterol fraction inhibited leukocyte granular enzyme release (beta -glucuronidase) and superoxide generation. However, it did not shown any. significant inhibitory effect on histamine release from mast cells. In vitro modulatory activity towards the classical pathway of the complement system shown by this fraction would correlate with the anti-inflammatory profile shown in vivo.

引用

页码：470 / 473

页数：4

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