Overexpression and involvement in migration by the metastasis-associated phosphatase PRL-3 in human myeloma cells

被引：88

作者：

Fagerli, Unn-Merete ^{[1
,5
]}

Holt, Randi U. ^{[1
,7
]}

Holien, Toril ^{[1
]}

Vaatsveen, Thea K. ^{[1
]}

Zhan, Fenghuang ^{[2
]}

Egeberg, Kjartan W. ^{[1
]}

Barlogie, Bart ^{[2
]}

Waage, Anders ^{[1
,6
]}

Aarset, Harald ^{[4
]}

Dai, Hong Yan ^{[1
,4
]}

Shaughnessy, John D., Jr. ^{[2
]}

Sundan, Anders ^{[1
]}

Borset, Magne ^{[1
,3
]}

机构：

[1] Norwegian Univ Sci & Technol, Dept Canc Res & Mol Med, N-7489 Trondheim, Norway

[2] Univ Arkansas Med Sci, Myeloma Inst Res & Therapy, Little Rock, AR 72205 USA

[3] St Olavs Univ Hosp, Dept Immunol & Transfus Med, Trondheim, Norway

[4] St Olavs Univ Hosp, Dept Pathol & Mol Genet, Trondheim, Norway

[5] St Olavs Univ Hosp, Dept Oncol, Trondheim, Norway

[6] St Olavs Univ Hosp, Dept Hematol, Trondheim, Norway

[7] Sor Trondelag Univ Coll, Fac Technol, Trondheim, Norway

来源：

BLOOD | 2008年 / 111卷 / 02期

关键词：

D O I：

10.1182/blood-2007-07-101139

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Multiple myeloma (MM) is characterized by accumulation and dissemination of malignant plasma cells (PCs) in the bone marrow (BM). Gene expression profiling of 2 MM cell lines (OH-2 and IH-1) indicated that expression of PRL-3, a metastasis-associated tyrosine phosphatase, was induced by several mitogenic cytokines. Cytokine-driven PRL-3 expression could be shown in several myeloma cell lines at both the mRNA and protein levels. There was significantly higher expression of the PRL-3 gene in PCs from patients with monoclonal gammopathy of undetermined significance (MGUS), smoldering myeloma (SMM), and myeloma than in PCs from healthy persons. Among 7 MM subgroups identified by unsupervised hierarchical cluster analysis, PRL-3 gene expression was significantly higher in the 3 groups denoted as "proliferation," "low bone disease," and "MMSET/FGFR3.," PRL-3 protein was detected in 18 of 20 BM biopsies from patients with MM. Silencing of the PRL-3 gene by siRNA reduced cell migration in the MM cell line INA-6, but had no detectable effect on proliferation and cell-cycle phase distribution of the cells. In conclusion, PRL-3 is a gene product specifically expressed in malignant plasma cells and may have a role in migration of these cells.

引用

页码：806 / 815

页数：10

共 56 条

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