Application of iron-tetrasulfonatophthalocyanine as a new mimetic peroxidase in the determination of hydrogen peroxide with p-hydroxyphenylpropionic acid as a substrate

被引：53

作者：

Chen, QY ^{[1
]}

Li, DH ^{[1
]}

Zhu, QZ ^{[1
]}

Zheng, H ^{[1
]}

Xu, JG ^{[1
]}

机构：

[1] Xiamen Univ, Dept Chem, Res Lab, SEDA Analyt Sci Mat & Life Chem, Xiamen 361005, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 1999年 / 381卷 / 2-3期

基金：

中国国家自然科学基金;

关键词：

mimetic enzyme; iron-tetrasulfonatophthalocyanine; fluorimetry; hydrogen peroxide determination;

D O I：

10.1016/S0003-2670(98)00743-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new promising mimetic peroxidase, iron-tetrasulfonatophthalocyanine (FeTSPC), has been synthesized and applied to the determination of hydrogen peroxide based on its catalytic effect on the oxidation of p-hydroxyphenylpropionic acid (p-HPPA). Under optimum conditions, the calibration graph has a linear range of 0.0x10(-6)-3.6x10(-6) mol/l hydrogen peroxide with a detection limit of 1.3x10(-8) mol/l in a 30 min reaction period. Application of the system to the determination of glucose shows a linear range 0.0x10(-6)-6.0x10(-6) mol/l and a detection limit of 3.1x10(-8) mol/l. The feasibility of FeTSPC as a new mimetic peroxidase in practical clinical analysis has been proven in the determination of glucose in human serum. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：175 / 182

页数：8

共 15 条

[1] SPECTROFLUORIMETRIC DETERMINATION OF HYDROGEN-PEROXIDE BASED ON THE CATALYTIC EFFECT OF PEROXIDASE-LIKE MANGANESE TETRAKIS(SULPHOPHENYL) PORPHYRIN ON THE OXIDATION OF HOMOVANILLIC-ACID [J].

CI, YX ;

WANG, F .

ANALYTICA CHIMICA ACTA, 1990, 233 (02) :299-302

[2]

CI YX, 1990, CHINESE J ANAL CHEM, V18, P334

[3]

GENFA Z, 1992, ANAL CHEM, V64, P517

[4] CHEMILUMINESCENCE ANALYSES OF BIOLOGICAL CONSTITUENTS USING METAL-COMPLEX CATALYSTS - A REVIEW [J].

HARA, T ;

TSUKAGOSHI, K .

ANALYTICAL SCIENCES, 1990, 6 (06) :797-806

[5] N,N-diethylaniline as hydrogen donor for determination of hydrogen peroxide catalyzed by metalloporphyrins as enzyme mimetic of peroxidase [J].

Huang, XM ;

Zhu, M ;

Shen, HX .

MIKROCHIMICA ACTA, 1998, 128 (1-2) :87-91

[6] Comparative study of some synthesised and commercial fluorogenic substrates for horseradish peroxidase and its mimetic enzyme hemin by a flow injection method [J].

Li, YZ ;

Townshend, A .

ANALYTICA CHIMICA ACTA, 1997, 340 (1-3) :159-168

[7] Iron-phthalocyanine immobilized on activated carbon black: A selective catalyst for alkane oxidation [J].

Parton, RF ;

Neys, PE ;

Jacobs, PA ;

Sosa, RC ;

Rouxhet, PG .

JOURNAL OF CATALYSIS, 1996, 164 (02) :341-346

[8]

QI YX, 1995, CHEM J CHINESE U, V16, P791

[9]

RAJA R, 1997, APPL CATAL A-GEN, V158, P7

[10]

SUN SS, 1990, CHIN J ANAL CHEM, V13, P329

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