Localisation and distribution of the major allergens in apple fruits

被引：106

作者：

Marzban, G

Puehringer, H

Dey, R

Brynda, S

Ma, Y

Martinelli, A

Zaccarini, M

van der Weg, E

Housley, Z

Kolarich, D

Altmann, F

Laimer, M

机构：

[1] BOKU, IAM, Plant Biotechnol Unit, Vienna, Austria

[2] Consorzio Italiano Vivaisti, Ferrara, Italy

[3] Plant Res Int, Wageningen, Netherlands

[4] Univ Basel, Biozentrum, Dept Biol Struct, CH-4003 Basel, Switzerland

[5] BOKU, Inst Chem, Vienna, Austria

来源：

PLANT SCIENCE | 2005年 / 169卷 / 02期

关键词：

Mal d 1 isoforms; Mal d 3; immuno-tissue-prints; ELISA; cultivar screening;

D O I：

10.1016/j.plantsci.2005.03.027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The importance of apple allergens, in particular Mal d 1, a Bet v 1 hornologue for the pollen-fruit syndrome in Northern Europe, and Mal d 3, responsible for true fruit allergy in Southern Europe, has been repeatedly emphasized. However, little is known about the distribution pattern of major allergens in fruits and whether differences exist among different cultivars. Transcript expression of Mal d 1 isoforms and Mal d 3 was examined by Real Time-PCR and Northern analysis, respectively. An immuno-tissue-print (ITP) assay was developed to localise major allergens in apple fruit tissue and a Mal d 1 specific, patient independent ELISA was established. ITP analyses show that Mal d 1 and Mal d 2 are distributed throughout the apple pulp and peel, while Mal d 3 is restricted to the peel. Data obtained by ELISA reveal a variation of Mal d 1 content ranging from 0.84 to 33.17 mu g/g fresh weight in 39 selected cultivars. Different apple cultivars show a markedly different expression of major allergens; this finding will influence the development of diagnostic tools as well as the dietary management of allergic individuals. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：387 / 394

页数：8

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