A new approach to manipulate the fate of single neural stem cells in tissue

被引：25

作者：

Taverna, Elena ^{[1
]}

Haffner, Christiane ^{[1
]}

Pepperkok, Rainer ^{[2
]}

Huttner, Wieland B. ^{[1
]}

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, Dresden, Germany

[2] European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, Germany

来源：

NATURE NEUROSCIENCE | 2012年 / 15卷 / 02期

基金：

欧洲研究理事会;

关键词：

RADIAL GLIA; IN-VIVO; NEUROEPITHELIAL CELLS; DEVELOPING NEOCORTEX; BASAL PROGENITORS; CEREBRAL-CORTEX; SELF-RENEWAL; NEURONS; NEUROGENESIS; EXPANSION;

D O I：

10.1038/nn.3008

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A challenge in the field of neural stem cell biology is the mechanistic dissection of single stem cell behavior in tissue. Although such behavior can be tracked by sophisticated imaging techniques, current methods of genetic manipulation do not allow researchers to change the level of a defined gene product on a truly acute time scale and are limited to very few genes at a time. To overcome these limitations, we established microinjection of neuroepithelial/radial glial cells (apical progenitors) in organotypic slice culture of embryonic mouse brain. Microinjected apical progenitors showed cell cycle parameters that were indistinguishable to apical progenitors in utero, underwent self-renewing divisions and generated neurons. Microinjection of single genes, recombinant proteins or complex mixtures of RNA was found to elicit acute and defined changes in apical progenitor behavior and progeny fate. Thus, apical progenitor microinjection provides a new approach to acutely manipulating single neural stem and progenitor cells in tissue.

引用

页码：329 / 337

页数：9

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