Cellular localization of group IIA phospholipase A2 in rats

被引：12

作者：

Yoshikawa, T

Naruse, S

Kitagawa, M

Ishiguro, H

Nagahama, M

Yasuda, E

Semba, R

Tanaka, M

Nomura, K

Hayakawa, T

机构：

[1] Nagoya Univ, Sch Med, Dept Internal Med 2, Showa Ku, Nagoya, Aichi 4668560, Japan

[2] Mie Univ, Sch Med, Dept Anat 2, Tsu, Mie 514, Japan

[3] Mie Univ, Sch Med, Dept Biochem, Tsu, Mie 514, Japan

[4] Shionogi Pharmaceut Co Ltd, Osaka, Japan

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2001年 / 49卷 / 06期

关键词：

group IIA phospholipase A(2); immunohistochemistry; in situ hybridization; rat; spleen; megakaryocyte; intestine; Paneth cell;

D O I：

10.1177/002215540104900611

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

It has been known that group II phospholipase A(2) (PLA(2)) mRNA and protein are present in the homogenates of the spleen, lung, liver, and kidney in normal rats, but the cellular origin of this enzyme has not been yet identified. At present, five subtypes of group II PLA(2) have been identified in mammals. Antibodies or mRNA probes previously used for detecting group II PLA(2) need to be evaluated to identify the subtypes of group II PLA(2). In this study we tried to identify group IIA PLA(2)-producing cells in normal rat tissues by in situ hybridization (ISH) using an almost full-length RNA probe for rat group IIA enzyme. Group IIA PLA(2) mRNA was detected in megakaryocytes in the spleen and Paneth cells in the intestine by ISH. These cells were also immunopositive for an antibody raised against group IIA PLA(2) isolated from rat platelets. Group IIA PLA2 mRNA-positive cells were not detected in lung, liver, kidney, and pancreas. Under normal conditions, group IIA PLA(2)-producing cells are splenic megakaryocytes and intestinal Paneth cells in rats.

引用

页码：777 / 782

页数：6

共 24 条

[1] Novel group V phospholipase A(2) involved in arachidonic acid mobilization in murine P388D(1) macrophages [J].