Separation of two constituents from purple sweet potato by combination of silica gel column and high-speed counter-current chromatography

被引：21

作者：

He, Kai ^{[1
,2
]}

Ye, Xiaoli ^{[1
,2
]}

Li, Xuegang ^{[3
]}

Chen, Hongying ^{[3
]}

Yuan, Lujiang ^{[3
]}

Deng, Yafei ^{[3
]}

Chen, Xin ^{[3
]}

Li, Xiaoduo ^{[1
,2
]}

机构：

[1] Southwest Univ, Sch Life Sci, Chongqing 400715, Peoples R China

[2] Chongqing Sweetpotato Res Ctr, Chongqing 400715, Peoples R China

[3] Southwest Univ, Coll Pharmaceut Sci, Chem Inst Pharmaceut Resources, Chongqing 400716, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2012年 / 881-82卷

基金：

国家科技攻关计划;

关键词：

Purple sweet potato; TLC-F method; High speed counter-current chromatography; Partition coefficient; Solvent system; BIOLOGICAL-ACTIVITIES; CHLOROGENIC ACIDS; ANTHOCYANINS; RATS; PURIFICATION; DERIVATIVES; INHIBITION; COUMARINS;

D O I：

10.1016/j.jchromb.2011.11.040

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

It is known that the choice of solvent system for high speed counter-current chromatography separation is of utmost importance. In this study, a simple and rapid thin layer chromatograph coupling with fluorometric (TLC-F) method has been used to determine the partition coefficient of target compounds in HSCCC solvent system. Two components, 6,7-dimethoxycoumarin and 5-hydroxymethy1-2-furfural were successfully separated from purple sweet potato extracts by successive sample injection for the first time, using n-hexane-ethyl acetate-methanol-water (1:2:1:1, v/v/v/v) as the solvent system. Additionally, statistical analysis showed that there was no significant difference in partition coefficient obtained by the TLC-F method and by HPLC, which demonstrated the usefulness of TLC-F method. (C) 2011 Elsevier BM. All rights reserved.

引用

页码：49 / 54

页数：6

共 32 条

[1]

Protective effects of coumarin and coumarin derivatives against carbon tetrachloride-induced acute hepatotoxicity in rats [J].