Application of liquid chromatography atmospheric pressure ionization mass spectrometry in natural product analysis - Evaluation and optimization of electrospray and heated nebulizer interfaces

被引：82

作者：

Zhou, SL ^{[1
]}

Hamburger, M ^{[1
]}

机构：

[1] NATL UNIV SINGAPORE, INST MOL & CELL BIOL, CTR NAT PROD RES, SINGAPORE 119260, SINGAPORE

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 755卷 / 02期

关键词：

interfaces; electrospray ionization; nebulizing interface; atmosphere pressure chemical ionization; detection; LC;

D O I：

10.1016/S0021-9673(96)00617-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A representative set of 46 secondary metabolites of plant and microbial origin was examined with electrospray and atmospheric pressure chemical ionization MS in both positive and negative ion mode, in order to evaluate the applicability of atmospheric pressure ionization LC-MS as a tool for rapid identification and dereplication of bioactive compounds in the drug screening process. MS characteristics of different compound classes were determined on the basis of quasi-molecular ion and other adduct ion responses observed with the two LC-MS interfaces. Interface parameters and LC mobile phase composition were optimized and an analytical protocol applicable to a wide range of secondary metabolites is proposed.

引用

页码：189 / 204

页数：16

共 48 条

[41] LIQUID-CHROMATOGRAPHIC UV DETECTION AND LIQUID-CHROMATOGRAPHIC THERMOSPRAY MASS-SPECTROMETRIC ANALYSIS OF CHIRONIA (GENTIANACEAE) SPECIES - A RAPID METHOD FOR THE SCREENING OF POLYPHENOLS IN CRUDE PLANT-EXTRACTS [J].

WOLFENDER, JL ;

HOSTETTMANN, K .

JOURNAL OF CHROMATOGRAPHY, 1993, 647 (02) :191-202

[42] SEARCH FOR BITTER PRINCIPLES IN CHIRONIA SPECIES BY LC-MS AND ISOLATION OF A NEW SECOIRIDOID DIGLYCOSIDE FROM CHIRONIA-KREBSII [J].

WOLFENDER, JL ;

HAMBURGER, M ;

HOSTETTMANN, K ;

MSONTHI, JD ;

MAVI, S .

JOURNAL OF NATURAL PRODUCTS, 1993, 56 (05) :682-689

[43] ELECTROSPRAY ION-SOURCE - ANOTHER VARIATION ON THE FREE-JET THEME [J].

YAMASHITA, M ;

FENN, JB .

JOURNAL OF PHYSICAL CHEMISTRY, 1984, 88 (20) :4451-4459

[44] GAS-PHASE BASICITIES AND PROTON AFFINITIES OF COMPOUNDS BETWEEN WATER AND AMMONIA AND SUBSTITUTED BENZENES FROM A CONTINUOUS LADDER OF PROTON-TRANSFER EQUILIBRIA MEASUREMENTS [J].

YAMDAGNI, R ;

KEBARLE, P .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1976, 98 (06) :1320-1324

[45] ISOLATION, STRUCTURES, AND ANTIFUNGAL ACTIVITIES OF NEW AUREOBASIDINS [J].

YOSHIKAWA, Y ;

IKAI, K ;

UMEDA, Y ;

OGAWA, A ;

TAKESAKO, K ;

KATO, I .

JOURNAL OF ANTIBIOTICS, 1993, 46 (09) :1347-1354

[46]

Zhou S., UNPUB

[47] Effects of solvent composition on molecular ion response in electrospray mass spectrometry: Investigation of the ionization processes [J].

Zhou, SL ;

Hamburger, M .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1995, 9 (15) :1516-1521

[48]

1992, API BOOK

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