Nickel content dependence of electrochemical behavior of carbohydrates on a titanium-nickel alloy electrode and its application to a liquid chromatography detector

被引：25

作者：

Morita, M

Niwa, O

Tou, S

Watanabe, N

机构：

[1] NTT, Basic Res Labs, Atsugi, Kanagawa 2430198, Japan

[2] BAS Japan Ltd, Tokyo 1318790, Japan

[3] Univ Tokyo, Fac Engn, Dept Appl Chem, Tokyo 1138656, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 837卷 / 1-2期

关键词：

electrochemical detection; detection; LC; nickel-titanium electrode; titanium-nickel electrode; electrodes; carbohydrates;

D O I：

10.1016/S0021-9673(99)00047-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive amperometric detector for the liquid chromatographic analysis of carbohydrates has been developed based on the optimization of the Ni content in a Ni-Ti alloy electrode. An optimum nickel content of 30 at. % was obtained from a signal-to-noise analysis in a flow injection experiment on glucose in 0.1 M NaOH. An optimum working potential of 0.5 V vs. Ag/AgCl was obtained from a hydrodynamic voltammogram. The glucose detection limit in the flow injection analysis was 11 fmol. The electrode was applied to an LC detector with a microbore column and the detection limit was 50 fmol. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：17 / 24

页数：8