A strategy for the sequential patterning of proteins: Catalytically active multiprotein nanofabrication

被引:25
作者
Jang, CH
Stevens, BD
Phillips, R
Calter, MA [1 ]
Ducker, WA
机构
[1] Virginia Tech, Dept Chem, Blacksburg, VA 24061 USA
[2] Univ Rochester, Dept Chem, Rochester, NY 14627 USA
关键词
D O I
10.1021/nl0259636
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We describe a new and simple method for immobilizing enzymes that will allow the preparation of thin films containing several different catalytically active enzymes. We use this method to immobilize acetylcholine esterase, then we show that its catalytic activity is retained after immobilizing a different protein on the same film.
引用
收藏
页码:691 / 694
页数:4
相关论文
共 13 条
[11]  
ROSENBERRY TL, 1975, ADV ENZYMOL RAMB, V43, P103
[12]   SPECIES VARIATION IN AMINO ACID SEQUENCE OF INSULIN [J].
SMITH, LF .
AMERICAN JOURNAL OF MEDICINE, 1966, 40 (05) :662-&
[13]   Fabrication and imaging of nanometer-sized protein patterns [J].
Wadu-Mesthrige, K ;
Xu, S ;
Amro, NA ;
Liu, GY .
LANGMUIR, 1999, 15 (25) :8580-8583