Cooperation of endoderm-derived BMP2 and extraembryonic ectoderm-derived BMP4 in primordial germ cell generation in the mouse

被引:296
作者
Ying, Y [1 ]
Zhao, GQ
机构
[1] Univ Missouri, Coll Vet Med, Dept Pathobiol, Columbia, MO 65211 USA
[2] Univ Texas, SW Med Ctr, Green Ctr Reprod Biol Sci, Dallas, TX 75390 USA
[3] Univ Texas, SW Med Ctr, Dept Pharmacol, Dallas, TX 75390 USA
关键词
Bmp2; Bmp4; Bmp8b; primordial germ cell; allantois; endoderm; mouse embryo;
D O I
10.1006/dbio.2001.0173
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The primordial germ cells (PGCs) of the mouse are derived from proximal epiblast cells that are adjacent to the extraembryonic ectoderm during gastrulation. Previous studies have demonstrated that extraembryonic ectoderm-derived BMP4 and BMP8B are both required for PGC generation. Here we show that Bmp2, a member of the Dpp class of the Bmp superfamily, also plays a role in PGC generation. PGC number is significantly reduced in Bmp2 heterozygous and homozygous embryos at the N2 generation onto C57BL/6, background. Bmp2 homozygous embryos also have a short allantois and about 50% of them do not undergo normal chorioallantoic fusion. Using whole-mount in situ hybridization, we show that Bmp2 is primarily expressed in the endoderm of mouse pregastrula and gastrula embryos. Using a genetic approach, we further show that Bmp2 and Bmp4, but not Bmp2 and Bmp8b, have an additive effect on PGC generation. These results suggest that PGC generation in the mouse embryo is regulated not only by extraembryonic ectoderm-derived BMP4 and BMP8B, but also by endoderm-derived BMP2. (C) 2001 Academic Press.
引用
收藏
页码:484 / 492
页数:9
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