Characterisation of a new rubredoxin isolated from Desulfovibrio desulfuricans 27774:: definition of a new family of rubredoxins

被引：24

作者：

LeGall, J

Liu, MY

Gomes, CM

Braga, V

Pacheco, I

Regalla, M

Xavier, AV

Teixeira, M

机构：

[1] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780 Oeiras, Portugal

[2] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA

来源：

FEBS LETTERS | 1998年 / 429卷 / 03期

关键词：

rubredoxin; rubrerythrin; Desulfovibrio; electron paramagnetic resonance; redox property;

D O I：

10.1016/S0014-5793(98)00610-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new rubredoxin from the sulphate-reducing bacterium Desulfovibrio desulfuvicans ATCC 27774, grown with nitrate as terminal electron acceptor, was isolated and characterised, The protein is an 8.5 kDa monomer containing one iron atom per molecule, with a reduction potential of 25 +/- 5 mV at pH 7.6. Like the recombinant Rdl protein from D. vulgaris, expressed in Escherichia coli [Lumpio, H.L., Shenvi, N.V., Garg, R.P., Summers. A.O. and Kurtz, D.M., J. Bacteriol. 179 (1997) 4607-1615], it contains an unusual spacing of four amino acids between the first two of the iron coordinating cysteinyl residues. This difference is reflected in the structure of the iron centre, as observed by visible and EPR spectroscopies. All together, these features make these proteins the first members of a new family of rubredoxins. (C) 1998 Federation of European Biochemical Societies.

引用

页码：295 / 298

页数：4

共 29 条

[11] Studies on the redox centers of the terminal oxidase from Desulfovibrio gigas and evidence for its interaction with rubredoxin [J].

Gomes, CM ;

Silva, G ;

Oliveira, S ;

LeGall, J ;

Liu, MY ;

Xavier, AV ;

RodriguesPousada, C ;

Teixeira, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (36) :22502-22508

[12] EPR SPECTROSCOPY OF IRON-SULFUR PROTEINS [J].

HAGEN, WR .

ADVANCES IN INORGANIC CHEMISTRY, 1992, 38 :165-222

[13] Structural and functional aspects of metal sites in biology [J].

Holm, RH ;

Kennepohl, P ;

Solomon, EI .

CHEMICAL REVIEWS, 1996, 96 (07) :2239-2314

[14] AMINO-ACID-SEQUENCE OF RUBREDOXIN FROM DESULFOVIBRIO-DESULFURICANS STRAIN 27774 [J].

HORMEL, S ;

WALSH, KA ;

PRICKRIL, BC ;

TITANI, K ;

LEGALL, J ;

SIEKER, LC .

FEBS LETTERS, 1986, 201 (01) :147-150

[15] The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus [J].

Klenk, HP ;

Clayton, RA ;

Tomb, JF ;

White, O ;

Nelson, KE ;

Ketchum, KA ;

Dodson, RJ ;

Gwinn, M ;

Hickey, EK ;

Peterson, JD ;

Richardson, DL ;

Kerlavage, AR ;

Graham, DE ;

Kyrpides, NC ;

Fleischmann, RD ;

Quackenbush, J ;

Lee, NH ;

Sutton, GG ;

Gill, S ;

Kirkness, EF ;

Dougherty, BA ;

McKenney, K ;

Adams, MD ;

Loftus, B ;

Peterson, S ;

Reich, CI ;

McNeil, LK ;

Badger, JH ;

Glodek, A ;

Zhou, LX ;

Overbeek, R ;

Gocayne, JD ;

Weidman, JF ;

McDonald, L ;

Utterback, T ;

Cotton, MD ;

Spriggs, T ;

Artiach, P ;

Kaine, BP ;

Sykes, SM ;

Sadow, PW ;

DAndrea, KP ;

Bowman, C ;

Fujii, C ;

Garland, SA ;

Mason, TM ;

Olsen, GJ ;

Fraser, CM ;

Smith, HO ;

Woese, CR .

NATURE, 1997, 390 (6658) :364-&

[16] SPECTROPHOTOMETRIC AND TURBIDIMETRIC METHODS FOR MEASURING PROTEINS [J].

LAYNE, E .

METHODS IN ENZYMOLOGY, 1957, 3 :447-454

[17] ISOLATION AND CHARACTERIZATION OF RUBRERYTHRIN, A NON-HEME IRON PROTEIN FROM DESULFOVIBRIO-VULGARIS THAT CONTAINS RUBREDOXIN CENTERS AND A HEMERYTHRIN-LIKE BINUCLEAR IRON CLUSTER [J].

LEGALL, J ;

PRICKRIL, BC ;

MOURA, I ;

XAVIER, AV ;

MOURA, JJG ;

HUYNH, BH .

BIOCHEMISTRY, 1988, 27 (05) :1636-1642

[18]

LIU MC, 1981, J BIOL CHEM, V256, P3159

[19] A rubrerythrin operon and nigerythrin gene in Desulfovibrio vulgaris (Hildenborough) [J].

Lumppio, HL ;

Shenvi, NV ;

Garg, RP ;

Summers, AO ;

Kurtz, DM .

JOURNAL OF BACTERIOLOGY, 1997, 179 (14) :4607-4615

[20]

MARTIN PS, 1988, P NATL ACAD SCI USA, V85, P9378

← 1 2 3 →