Diagonal chromatographic selection of cysteinyl peptides modified with benzoquinones

被引：4

作者：

Dayon, Loic ^{[1
]}

Girault, Hubert H. ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Electrochim Phys & Analyt, CH-1015 Lausanne, Switzerland

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 389卷 / 03期

关键词：

COFRADIC (TM); labeling; liquid chromatography; mass spectrometry; proteomics; tagging;

D O I：

10.1007/s00216-007-1492-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The derivatization of cysteine-containing peptides with benzoquinone compounds is rapid, quantitative and specific in acidic media. The conversion of cysteines into hydrophobic benzoquinone-adducted residues in peptides is used here to alter the chromatographic properties of cysteinyl peptides during liquid chromatography separation. The benzoquinone derivatization is shown to allow the accurate selection of cysteine-containing peptides of bovine serum albumin tryptic digest by diagonal reversed-phase chromatography, which consists of one primary and a series of secondary identical liquid chromatographic separations, before and after a cysteinyl-targeted modification of the peptides by benzoquinone compounds.

引用

页码：841 / 849

页数：9

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