Creatine analogue beta-guanidinopropionic acid alters skeletal muscle AMP deaminase activity

被引:19
作者
Tullson, PC [1 ]
Rundell, KW [1 ]
Sabina, RL [1 ]
Terjung, RL [1 ]
机构
[1] MED COLL WISCONSIN, DEPT BIOCHEM, MILWAUKEE, WI 53226 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1996年 / 270卷 / 01期
关键词
inosine 5'-monophosphate; deamination; adenine nucleotides; enzyme regulation; purine nucleotide cycle;
D O I
10.1152/ajpcell.1996.270.1.C76
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Dietary supplementation of the creatine analogue beta-guanidinopropionic acid (beta-CTPA) decreases in vitro skeletal muscle AMP deaminase (AMP-D) activity in rats. Downregulation of AMP-D activity was progressive and greater in fast-twitch muscles (70-80%) than in the slow-twitch soleus muscle (similar to 50%). The loss in AMP-D activity had little effect on inosine 5'-monophosphate accumulation in mixed-fiber muscle with intense tetanic contractions. In contrast, inosine 5'-monophosphate formation was evident earlier in fast-twitch red and white fiber sections of creatine-depleted animals during intense twitch contractions, indicating that fast-twitch muscle of beta-GPA-treated rats buffers decreases in the ATP/ADP(free) ratio via deamination, even though AMP-D activity is less. Isoforms of skeletal muscle AMP-D mRNAs in mixed-fiber muscle were not altered by feeding beta-GPA for up to 9 wk. Creatine depletion did not alter total immunoreactivity; however, a redistribution of AMP-D immunoreactivity from primarily an similar to 80-kDa form toward lower apparent molecular mass species (similar to 60 and similar to 56 kDa) was observed. Posttranslational changes in AMP-D appear related to changes in activity.
引用
收藏
页码:C76 / C85
页数:10
相关论文
共 39 条
  • [1] EXTRACTION PROCEDURES FOR USE PRIOR TO HPLC NUCLEOTIDE ANALYSIS USING MICROPARTICLE CHEMICALLY BONDED PACKINGS
    CHEN, SC
    BROWN, PR
    ROSIE, DM
    [J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1977, 15 (06) : 218 - 221
  • [2] BETA-GUANIDINOPROPIONATE AND PHOSPHORYLATED BETA-GUANIDINOPROPIONATE AS SUBSTRATES FOR CREATINE-KINASE
    CHEVLI, R
    FITCH, CD
    [J]. BIOCHEMICAL MEDICINE, 1979, 21 (02): : 162 - 167
  • [3] DUDLEY GA, 1987, J BIOL CHEM, V262, P9109
  • [4] DUDLEY GA, 1985, AM J PHYSIOL, V248, pC43
  • [5] SUSTAINED ISOMETRIC CONTRACTION OF SKELETAL-MUSCLE DEPLETED OF PHOSPHOCREATINE
    FITCH, CD
    CHEVLI, R
    PETROFSKY, JS
    KOPP, SJ
    [J]. LIFE SCIENCES, 1978, 23 (12) : 1285 - 1291
  • [6] PHOSPHORYLATED BETA-GUANIDINOPROPIONATE AS A SUBSTITUTE FOR PHOSPHOCREATINE IN RAT MUSCLE
    FITCH, CD
    JELLINEK, M
    FITTS, RH
    BALDWIN, KM
    HOLLOSZY, JO
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1975, 228 (04): : 1123 - 1125
  • [7] MEASUREMENT OF BETA-GUANIDINOPROPIONATE AND PHOSPHORYLATED BETA-GUANIDINOPROPIONATE IN TISSUES
    FITCH, CD
    CHEVLI, R
    [J]. ANALYTICAL BIOCHEMISTRY, 1975, 68 (01) : 196 - 201
  • [8] DIFFERENT EFFECTS OF GRADUAL VS ACUTE ADENINE-NUCLEOTIDE DEPLETION ON ATP COST OF MUSCLE-CONTRACTION
    FOLEY, JM
    ADAMS, GR
    MEYER, RA
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1994, 267 (05): : C1177 - C1184
  • [9] GORI Z, 1988, BRIT J EXP PATHOL, V69, P639
  • [10] OXYGEN COST OF TWITCH AND TETANIC ISOMETRIC CONTRACTIONS OF RAT SKELETAL-MUSCLE
    HOOD, DA
    GORSKI, J
    TERJUNG, RL
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1986, 250 (04): : E449 - E456