Imaging plasmodesmata

被引:42
作者
Bell, Karen [1 ]
Oparka, Karl [1 ]
机构
[1] Univ Edinburgh, Inst Mol Plant Sci, Edinburgh EH9 3JR, Midlothian, Scotland
基金
英国生物技术与生命科学研究理事会;
关键词
Plasmodesmata; Transmission electron microscopy; 3D-structured illumination microscopy; VIRUS MOVEMENT PROTEIN; SECONDARY PLASMODESMATA; ELECTRON-MICROSCOPY; ULTRASTRUCTURAL ASPECTS; ENDOPLASMIC-RETICULUM; LIVING CELLS; FLUORESCENCE; LIGHT; GFP; 3D;
D O I
10.1007/s00709-010-0233-6
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
At only 50 nm in diameter, plasmodesmata (PD) are below the limit of resolution of conventional light microscopy. Consequently, much of our current interpretation of the substructure of PD is derived from transmission electron microscopy. However, PD can be imaged with alternative techniques, including field emission scanning electron microscopy and 'super-resolution' imaging approaches such as 3D-structured illumination microscopy. This review considers the methods currently available for studying PD and focuses on the boundary between light- and electron-based imaging approaches.
引用
收藏
页码:9 / 25
页数:17
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