High-resolution in vivo imaging of blood vessels without labeling

被引：100

作者：

Fu, Dan ^{[1
]}

Ye, Tong

Matthews, Thomas E.

Chen, Benny J.

Yurtserver, Gunay

Warren, Warren S.

机构：

[1] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

[2] Duke Univ, Dept Chem, Durham, NC 27705 USA

[3] Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA

[4] Duke Univ, Dept Biomed Engn, Durham, NC 27705 USA

来源：

OPTICS LETTERS | 2007年 / 32卷 / 18期

关键词：

D O I：

10.1364/OL.32.002641

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate that both oxyhemoglobin and deoxyhemoglobin have sequential two-color, two-photon absorption properties that can serve as endogenous contrasts in microvasculature imaging. Using a sensitive modulation transfer technique, we are able to image hemoglobin in red blood cells with micrometer resolution, both in vitro and in vivo. We show that excellent contrast from hemoglobin without any labeling can be obtained in tissue. (c) 2007 Optical Society of America.

引用

收藏

页码：2641 / 2643

页数：3

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