Nucleosomes represent a physical barrier for cleavage activity of DNA topoisomerase I in vivo

被引：8

作者：

Di Felice, Francesca ^{[1
]}

Chiani, Francesco ^{[1
]}

Camilloni, Giorgio ^{[1
,2
]}

机构：

[1] Univ Roma La Sapienza, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy

[2] CNR, Ist Biol & Patol Mol, I-00185 Rome, Italy

来源：

BIOCHEMICAL JOURNAL | 2008年 / 409卷 / 03期

关键词：

chromatin; DNA structure; DNA topoisomerase I; nucleosome; Saccharomyces cerevisiae; trinucleotide repeats;

D O I：

10.1042/BJ20070893

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

DNA topoisomerase I together with the other cellular DNA topoisomerases releases the torsional stress from DNA caused by processes such as replication, transcription and recombination. Despite the well-defined knowledge of its mechanism of action, DNA topoisomerase I in vivo activity has been only partially characterized. In fact the basic question concerning the capability of the enzyme to cleave and rejoin DNA wrapped around a histone octamer remains still unanswered. By studying both in vivo and in vitro the cleavage activity of DNA topoisomerase I in the presence of camptothecin on a repeated trinucleotide sequence, (TTA)(35), lying in chromosome XIII of Saccharomyces cerevisiae, we can conclude that nucleosomes represent a physical barrier for the enzyme activity.

引用

页码：651 / 656

页数：6

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