Effect of some physico-chemical conditions on an immunoassay for viable Escherichia coli

被引：5

作者：

Jurkevica, Agnese ^{[1
]}

Halsall, H. Brian ^{[1
]}

Seliskar, Carl J. ^{[1
]}

Heineman, William R. ^{[1
]}

机构：

[1] Univ Cincinnati, Dept Chem, Cincinnati, OH 45221 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 397卷 / 07期

基金：

美国国家科学基金会;

关键词：

Escherichia coli; Immunoassay; Fluorescence detection; Effect of physico-chemical conditions; COATED PARAMAGNETIC BEADS; D-GALACTOSIDASE ACTIVITY; O157-H7; GROWTH; DIFFERENCE; PARAMETERS; STRAINS; WATER; HEAT; LIVE;

D O I：

10.1007/s00216-010-3846-x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Viable Escherichia coli can be detected by an immunoassay in which live bacteria captured on antibody-coated paramagnetic beads are induced to synthesize the enzyme beta-galactosidase, which catalyzes the hydrolysis of the slightly fluorescent substrate 4-methyl umbelliferyl-beta-d-galactoside to the highly fluorescent product 7-hydroxy-4-methylcoumarin for detection. The effects of bacterial strain, presence of dead bacteria, and some environmental stresses on assay performance were evaluated.

引用

页码：3133 / 3136

页数：4

共 17 条

[1] Amperometric determination of live Escherichia coli using antibody-coated paramagnetic beads [J].

Boyaci, IH ;

Aguilar, ZP ;

Hossain, M ;

Halsall, HB ;

Seliskar, CJ ;

Heineman, WR .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005, 382 (05) :1234-1241

[2]

DALBOW DG, 1975, BIOCHEM J, V150, P13, DOI 10.1042/bj1500013

[3] Determination of viable Escherichia coli using antibody-coated paramagnetic beads with fluorescence detection [J].

Dudak, F. Ceyda ;

Boyaci, Ismail H. ;

Jurkevica, Agnese ;

Hossain, Mahmud ;

Aquilar, Zoraida ;

Halsall, H. Brian ;

Seliskar, Carl J. ;

Heineman, William R. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2009, 393 (03) :949-956

[4]

DWORKIN M, 2007, PROKARYOTES SYMBIOTI

[5] Effect of u.v. light irradiation, starvation and heat on Escherichia coli β-D-galactosidase activity and other potential viability parameters [J].

Fiksdal, L ;

Tryland, I .

JOURNAL OF APPLIED MICROBIOLOGY, 1999, 87 (01) :62-71

[6] Effect of environmental stresses on antibody-based detection of Escherichia coli O157: H7, Salmonella enterica serotype Enteritidis and Listeria monocytogenes [J].

Hahm, B. -K. ;

Bhunia, A. K. .

JOURNAL OF APPLIED MICROBIOLOGY, 2006, 100 (05) :1017-1027

[7] Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110 [J].

Hayashi, Koji ;

Morooka, Naoki ;

Yamamoto, Yoshihiro ;

Fujita, Katsutoshi ;

Isono, Katsumi ;

Choi, Sunju ;

Ohtsubo, Eiichi ;

Baba, Tomoya ;

Wanner, Barry L. ;

Mori, Hirotada ;

Horiuchi, Takashi .

MOLECULAR SYSTEMS BIOLOGY, 2006, 2 (1) :2006.0007

[8] Advanced wastewater disinfection technologies: Short and long term efficiency [J].

Lazarova, V ;

Janex, ML ;

Fiksdal, L ;

Oberg, C ;

Barcina, I ;

Pommepuy, M .

WATER SCIENCE AND TECHNOLOGY, 1998, 38 (12) :109-117

[9] Reduced outer membrane permeability of Escherichia coli O157:H7:: Suggested role of modified outer membrane porins and theoretical function in resistance to antimicrobial agents [J].

Martinez, MB ;

Flickinger, M ;

Higgins, L ;

Krick, T ;

Nelsestuen, GL .

BIOCHEMISTRY, 2001, 40 (40) :11965-11974

[10] β-galactosidase activity of Escherichia coli under long-term starvation, alterations in temperature, and different nutrient conditions in lake water [J].

Özkanca R. .

International Microbiology, 2002, 5 (3) :127-132

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