Specificity of an Aspergillus niger esterase deacetylating cellulose acetate

被引：22

作者：

Altaner, C ^{[1
]}

Saake, B ^{[1
]}

Puls, H ^{[1
]}

机构：

[1] Fed Res Ctr Forestry & Forest Prod, Fed Res Ctr, Inst Wood Chem & Chem Technol Wood, D-21031 Hamburg, Germany

来源：

CELLULOSE | 2003年 / 10卷 / 01期

关键词：

acetylated xylan; acetyl esterase; Aspergillus niger; cellulose acetate; endoglucanase; regioselective deacetylation; substrate inhibition;

D O I：

10.1023/A:1023010330865

中图分类号：

TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];

学科分类号：

0805 ; 080502 ; 0822 ;

摘要：

A purified acetyl esterase (AE), isolated from a commercial enzyme preparation, released acetic acid from water-soluble and water-insoluble cellulose acetates (CAs), native and chemically acetylated xylan as well as acetylated starch. The AE specifically cleaved off the acetyl substituents from the C-2- and C-3-positions from CAs of DS < 1.8 and left the acetyl substituents at the C-6-positions intact without degrading the polysaccharide. The activity of endoglucanase was enhanced by the presence of acetyl esterase, while the acetyl esterase derived no advantage from the presence of the endoglucanase; it was able to function independently.

引用

页码：85 / 95

页数：11

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