Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples

被引：94

作者：

Hua, Xiude ^{[1
]}

Qian, Guoliang ^{[1
]}

Yang, Jifei ^{[2
]}

Hu, Baishi ^{[1
]}

Fan, Jiaqin ^{[1
]}

Qin, Na ^{[1
]}

Li, Gang ^{[1
]}

Wang, Yuyan ^{[1
]}

Liu, Fengquan ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Plant Protect, Chinese Minist Agr, Key Lab Monitoring & Management Plant Dis & Insec, Nanjing 210095, Peoples R China

[2] Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450000, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2010年 / 26卷 / 01期

基金：

国家高技术研究发展计划(863计划);

关键词：

Chlorpyrifos-methyl; Gold immunochromatographic assay; Colloidal gold; One-step strip test; Pesticide residue; ORGANOPHOSPHOROUS PESTICIDE-RESIDUES; LINKED IMMUNOSORBENT ASSAYS; MONOCLONAL-ANTIBODIES; LIQUID-CHROMATOGRAPHY; PARATHION-METHYL; REAL SAMPLES; ORGANOCHLORINE; SPECTROMETRY; IMMUNOASSAY; PERFORMANCE;

D O I：

10.1016/j.bios.2010.06.005

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A rapid (less than 10 min), qualitative and semi-quantitative immunochromatography using colloidal gold-antibody probe was successfully developed and applied in determination of chlorpyrifos-methyl (a wide-spectrum organophosphorus pesticide) in water samples The qualitative detection limit of chlorpyrifos-methyl was determined as 0 6 mu g ml(-1) by using immunochromatography. In the semi-quantitative experiment, the detection results of chlorpyrifos-methyl were scanned by a membrane strip reader, and a detection curve representing the scanned data average was obtained. After conversion, it was observed that in the range of 50 - 12,150 ng ml(-1), the graph between logit(B/B-0)and logarithm of concentration of chlorpyrifos-methyl was linear, from which, the regression equation (y = 2 5229x + 7 5951, R-2 = 0 9889) and IC50 value (1024.39 ng ml(-1)) was obtained, respectively Meanwhile, the detection limit was calculated as 132 91 ng ml(-1) by the extrapolation of B-0 - 2SD In addition, the cross-reactivines were less than 1% with tested analog compounds and regarded as negligible The recoveries obtained by standard chlorpyrifos-methyl addition to water samples were 102 5-107 6% Overall, to our knowledge, this is the first report of qualitative and semi-quantitative detection of chlorpyrifos-methyl by immunochromatography (C) 2010 Elsevier B V All rights reserved.

引用

页码：189 / 194

页数：6

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