Nuclease stability of LNA oligonucleotides and LNA-DNA chimeras

被引:52
作者
Frieden, M [1 ]
Hansen, HF [1 ]
Koch, T [1 ]
机构
[1] Cureon AS, DK-2100 Copenhagen, Denmark
关键词
LNA; alpha-L-LNA; nuclease stability;
D O I
10.1081/NCN-120022731
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The stabilizing properties of LNA and alpha-L-LNA oligonucleotides against endo- and 3'-exonucleases have been evaluated.
引用
收藏
页码:1041 / 1043
页数:3
相关论文
共 4 条
[1]   LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition [J].
Koshkin, AA ;
Singh, SK ;
Nielsen, P ;
Rajwanshi, VK ;
Kumar, R ;
Meldgaard, M ;
Olsen, CE ;
Wengel, J .
TETRAHEDRON, 1998, 54 (14) :3607-3630
[2]   Stability and structural features of the duplexes containing nucleoside analogues with a fixed N-type conformation, 2′-O,4′-C-methyleneribonucleosides [J].
Obika, S ;
Nanbu, D ;
Hari, Y ;
Andoh, J ;
Morio, K ;
Doi, T ;
Imanishi, T .
TETRAHEDRON LETTERS, 1998, 39 (30) :5401-5404
[3]   LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition [J].
Singh, SK ;
Nielsen, P ;
Koshkin, AA ;
Wengel, J .
CHEMICAL COMMUNICATIONS, 1998, (04) :455-456
[4]   Potent and nontoxic antisense oligonucleotides containing locked nucleic acids [J].
Wahlestedt, C ;
Salmi, P ;
Good, L ;
Kela, J ;
Johnsson, T ;
Hökfelt, T ;
Broberger, C ;
Porreca, F ;
Lai, J ;
Ren, KK ;
Ossipov, M ;
Koshkin, A ;
Jakobsen, N ;
Skouv, J ;
Oerum, H ;
Jacobsen, MH ;
Wengel, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (10) :5633-5638