Identification of the connecting linkage between homo- or xylogalacturonan and rhamnogalacturonan type I

被引：127

作者：

Coenen, G. J. ^{[1
]}

Bakx, E. J. ^{[1
]}

Verhoef, R. P. ^{[1
]}

Schols, H. A. ^{[1
]}

Voragen, A. G. J. ^{[1
]}

机构：

[1] Wageningen Univ, Dept Agrotechnol & Food Sci, Food Chem Lab, NL-6700 EV Wageningen, Netherlands

来源：

CARBOHYDRATE POLYMERS | 2007年 / 70卷 / 02期

关键词：

homogalacturonan; xylogalacturonan; rhamnogalacturonan I; pectin model; covalent linkage; pectin structure;

D O I：

10.1016/j.carbpol.2007.04.007

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Pectin is of interest both as cell wall component and as food additive. The precise chemical structure of pectin remains under debate, although the structural elements of pectin are rather well described. In order to get more insight in the inter linkage between the various structural elements, apple pectin modified hairy regions were degraded by controlled acid hydrolysis. From the degradation products oligomeric fragments were selected which could represent interconnection points, and these oligomers were characterized using LCMS and NMR approaches. It was shown that the oligomers GalA(3)Rha(1), GalA(4)Rha(2) and GalA(5)Rha(3) consisted out of a homogalacturonan and a rhamnogalacturonan type I segment connected via a GalAp alpha-(1 -> 2) Rhap linkage. In addition, a GalA(6)Rha(3)Xyl(1) oligomer was identified, which consisted out of a xylogalacturonan and a rhamnogalacturonan type I segment. These oligomers indicated that in apple pectin both homogalacturonan and xylogalacturonan were covalently linked to rhamnogalacturonan type I. With these new insights, currently used pectin models were refined. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：224 / 235

页数：12

共 66 条

[31] STUDIES ON THE INTERMOLECULAR DISTRIBUTION OF INDUSTRIAL PECTINS BY MEANS OF PREPARATIVE SIZE EXCLUSION CHROMATOGRAPHY [J].

KRAVTCHENKO, TP ;

BERTH, G ;

VORAGEN, AGJ ;

PILNIK, W .

CARBOHYDRATE POLYMERS, 1992, 18 (04) :253-263

[32] TREATMENT OF RHAMNOGALACTURONAN-I WITH LITHIUM IN ETHYLENEDIAMINE [J].

LAU, JM ;

MCNEIL, M ;

DARVILL, AG ;

ALBERSHEIM, P .

CARBOHYDRATE RESEARCH, 1987, 168 (02) :245-274

[33] Extraction, purification and chemical characterisation of xylogalacturonans from pea hulls [J].

Le Goff, A ;

Renard, CMGC ;

Bonnin, E ;

Thibault, JF .

CARBOHYDRATE POLYMERS, 2001, 45 (04) :325-334

[34]

May C. D., 2000, Handbook of hydrocolloids, P169

[35] STRUCTURE OF PLANT-CELL WALLS .10. RHAMNOGALACTURONAN-I, A STRUCTURALLY COMPLEX PECTIC POLYSACCHARIDE IN THE WALLS OF SUSPENSION-CULTURED SYCAMORE CELLS [J].

MCNEIL, M ;

DARVILL, AG ;

ALBERSHEIM, P .

PLANT PHYSIOLOGY, 1980, 66 (06) :1128-1134

[36]

Mohnen D., 1999, COMPREHENSIVE NATURA, V3, P497

[37] Characterization of recombinant rhamnogalacturonan α-L-rhamnopyranosyl-(1,4)-α-D-galactopyranosyluronide lyase from Aspergillus aculeatus -: An enzyme that fragments rhamnogalacturonan I regions of pectin [J].

Mutter, M ;

Colquhoun, IJ ;

Beldman, G ;

Schols, HA ;

Bakx, EJ ;

Voragen, AGJ .

PLANT PHYSIOLOGY, 1998, 117 (01) :141-152

[38] Rhamnogalacturonan α-D-galactopyranosyluronohydrolase -: An enzyme that specifically removes the terminal nonreducing galacturonosyl residue in rhamnogalacturonan regions of pectin [J].

Mutter, M ;

Beldman, G ;

Pitson, SM ;

Schols, HA ;

Voragen, AGJ .

PLANT PHYSIOLOGY, 1998, 117 (01) :153-163

[39] Rhamnogalacturonan II: Structure and function of a borate cross-linked cell wall pectic polysaccharide [J].

O'Neill, MA ;

Ishii, T ;

Albersheim, P ;

Darvill, AG .

ANNUAL REVIEW OF PLANT BIOLOGY, 2004, 55 :109-139

[40] Requirement of borate cross-linking of cell wall rhamnogalacturonan II for Arabidopsis growth [J].

O'Neill, MA ;

Eberhard, S ;

Albersheim, P ;

Darvill, AG .

SCIENCE, 2001, 294 (5543) :846-849

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