Construction and rapid testing of synthetic and modified toxin gene sequences CryIA (b&c) by expression in maize endosperm culture

被引:60
作者
Sardana, R [1 ]
Dukiandjiev, S [1 ]
Giband, M [1 ]
Cheng, XY [1 ]
Cowan, K [1 ]
Sauder, C [1 ]
Altosaar, I [1 ]
机构
[1] UNIV OTTAWA,DEPT BIOCHEM,OTTAWA,ON K1N 6N5,CANADA
关键词
toxin genes; maize endosperm culture; PCR; ubiquitin promoter;
D O I
10.1007/BF00231923
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The synthesis of two modified genes, Cry IA(b) and CryIA(c), each consisting of 1845 bp, is described in detail. The genes were synthesized using an improved PCR procedure based on recursive principles. The synthetic CryIA(c) gene was put under the control of a maize ubiquitin promoter. This construct was tested in a maize endosperm-derived suspension culture system. The use of maize endosperm culture as a quick and efficient system to test the activity of synthetic genes is described.
引用
收藏
页码:677 / 681
页数:5
相关论文
共 18 条
[1]   THE RECONSTRUCTION AND EXPRESSION OF A BACILLUS-THURINGIENSIS CRYIIIA GENE IN PROTOPLASTS AND POTATO PLANTS [J].
ADANG, MJ ;
BRODY, MS ;
CARDINEAU, G ;
EAGAN, N ;
ROUSH, RT ;
SHEWMAKER, CK ;
JONES, A ;
OAKES, JV ;
MCBRIDE, KE .
PLANT MOLECULAR BIOLOGY, 1993, 21 (06) :1131-1145
[2]   MAIZE POLYUBIQUITIN GENES - STRUCTURE, THERMAL PERTURBATION OF EXPRESSION AND TRANSCRIPT SPLICING, AND PROMOTER ACTIVITY FOLLOWING TRANSFER TO PROTOPLASTS BY ELECTROPORATION [J].
CHRISTENSEN, AH ;
SHARROCK, RA ;
QUAIL, PH .
PLANT MOLECULAR BIOLOGY, 1992, 18 (04) :675-689
[3]   ACTIVITY OF A MAIZE UBIQUITIN PROMOTER IN TRANSGENIC RICE [J].
CORNEJO, MJ ;
LUTH, D ;
BLANKENSHIP, KM ;
ANDERSON, OD ;
BLECHL, AE .
PLANT MOLECULAR BIOLOGY, 1993, 23 (03) :567-581
[4]  
FUJIMOTO H, 1993, BIO-TECHNOL, V11, P1151, DOI 10.1038/nbt1093-1151
[5]  
JEFFERSON RA, 1987, EMBO J, V6, P3901
[6]   HIGH-VELOCITY MICROPROJECTILES FOR DELIVERING NUCLEIC-ACIDS INTO LIVING CELLS [J].
KLEIN, TM ;
WOLF, ED ;
WU, R ;
SANFORD, JC .
NATURE, 1987, 327 (6117) :70-73
[7]   FIELD PERFORMANCE OF ELITE TRANSGENIC MAIZE PLANTS EXPRESSING AN INSECTICIDAL PROTEIN DERIVED FROM BACILLUS-THURINGIENSIS [J].
KOZIEL, MG ;
BELAND, GL ;
BOWMAN, C ;
CAROZZI, NB ;
CRENSHAW, R ;
CROSSLAND, L ;
DAWSON, J ;
DESAI, N ;
HILL, M ;
KADWELL, S ;
LAUNIS, K ;
LEWIS, K ;
MADDOX, D ;
MCPHERSON, K ;
MEGHJI, MR ;
MERLIN, E ;
RHODES, R ;
WARREN, GW ;
WRIGHT, M ;
EVOLA, SV .
BIO-TECHNOLOGY, 1993, 11 (02) :194-200
[8]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[9]   MODIFICATION OF THE CODING SEQUENCE ENHANCES PLANT EXPRESSION OF INSECT CONTROL PROTEIN GENES [J].
PERLAK, FJ ;
FUCHS, RL ;
DEAN, DA ;
MCPHERSON, SL ;
FISCHHOFF, DA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (08) :3324-3328
[10]   INSECT RESISTANT COTTON PLANTS [J].
PERLAK, FJ ;
DEATON, RW ;
ARMSTRONG, TA ;
FUCHS, RL ;
SIMS, SR ;
GREENPLATE, JT ;
FISCHHOFF, DA .
BIO-TECHNOLOGY, 1990, 8 (10) :939-943