Construction and rapid testing of synthetic and modified toxin gene sequences CryIA (b&c) by expression in maize endosperm culture

被引：60

作者：

Sardana, R ^{[1
]}

Dukiandjiev, S ^{[1
]}

Giband, M ^{[1
]}

Cheng, XY ^{[1
]}

Cowan, K ^{[1
]}

Sauder, C ^{[1
]}

Altosaar, I ^{[1
]}

机构：

[1] UNIV OTTAWA,DEPT BIOCHEM,OTTAWA,ON K1N 6N5,CANADA

来源：

PLANT CELL REPORTS | 1996年 / 15卷 / 09期

关键词：

toxin genes; maize endosperm culture; PCR; ubiquitin promoter;

D O I：

10.1007/BF00231923

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The synthesis of two modified genes, Cry IA(b) and CryIA(c), each consisting of 1845 bp, is described in detail. The genes were synthesized using an improved PCR procedure based on recursive principles. The synthetic CryIA(c) gene was put under the control of a maize ubiquitin promoter. This construct was tested in a maize endosperm-derived suspension culture system. The use of maize endosperm culture as a quick and efficient system to test the activity of synthetic genes is described.

引用

页码：677 / 681

页数：5

共 18 条

[11] RECURSIVE PCR - A NOVEL TECHNIQUE FOR TOTAL GENE SYNTHESIS [J].