High-performance liquid chromatographic method for the determination of HIV-1 non-nucleoside reverse transcriptase inhibitor efavirenz in plasma of patients during highly active antiretroviral therapy

被引：42

作者：

Langmann, P ^{[1
]}

Schirmer, D ^{[1
]}

Väth, T ^{[1
]}

Zilly, M ^{[1
]}

Klinker, H ^{[1
]}

机构：

[1] Univ Wurzburg, Dept Internal Med, Div Infect Dis, D-97080 Wurzburg, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 755卷 / 1-2期

关键词：

efavirenz;

D O I：

10.1016/S0378-4347(01)00059-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new high-performance liquid chromatographic method for the determination of efavirenz in human plasma is described. Quantitative recovery following liquid-liquid extraction with diethylether from 200 mul of human plasma was achieved. Subsequently, the assay was performed with 67 mM potassium dihydrogen phosphate-acetonitrile as a mobile phase, a XTerraRP 18 column protected with a Phenomenex C-18 column and UV detection at 246 nm. Linear standard curves were obtained for concentrations ranging from 25 to 15 000 ng/ml. The calculated intra- and inter-day coefficients of variation were below 10%. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：151 / 156

页数：6

共 7 条

[1] Efavirenz [J].

Adkins, JC ;

Noble, S .

DRUGS, 1998, 56 (06) :1055-1064

[2] Determination of twelve antiretroviral agents in human plasma sample using reversed-phase high-performance liquid chromatography [J].

Aymard, G ;

Legrand, M ;

Trichereau, N ;

Diquet, B .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2000, 744 (02) :227-240

[3] High-performance liquid chromatographic method for the simultaneous determination of HIV-1 protease inhibitors indinavir, saquinavir and ritonavir in plasma of patients during highly active antiretroviral therapy [J].

Langmann, P ;

Klinker, H ;

Schirmer, D ;

Zilly, M ;

Bienert, A ;

Richter, E .

JOURNAL OF CHROMATOGRAPHY B, 1999, 735 (01) :41-50

[4] Simultaneous determination of the HIV protease inhibitors indinavir, amprenavir, saquinavir, ritonavir, nelfinavir and the non-nucleoside reverse transcriptase inhibitor efavirenz by high-performance liquid chromatography after solid-phase extraction [J].

Marzolini, C ;

Telenti, A ;

Buclin, T ;

Biollaz, J ;

Decosterd, LA .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2000, 740 (01) :43-58

[5] Rapid quantification of delavirdine, a novel non-nucleoside reverse transcriptase inhibitor, in human plasma using isocratic reversed-phase high-performance liquid chromatography with fluorescence detection [J].

Veldkamp, AI ;

van Heeswijk, RPG ;

Hoetelmans, RMW ;

Meenhorst, PL ;

Mulder, JW ;

Lange, JMA ;

Beijnen, JH .

JOURNAL OF CHROMATOGRAPHY B, 1999, 727 (1-2) :151-157

[6] Quantitative determination of efavirenz (DMP 266), a novel non-nucleoside reverse transcriptase inhibitor, in human plasma using isocratic reversed-phase high-performance liquid chromatography with ultraviolet detection [J].

Veldkamp, AI ;

van Heeswijk, RPG ;

Meenhorst, PL ;

Mulder, JW ;

Lange, JMA ;

Beijnen, JH ;

Hoetelmans, RMW .

JOURNAL OF CHROMATOGRAPHY B, 1999, 734 (01) :55-61

[7] High-performance liquid chromatography method for analyzing the antiretroviral agent efavirenz in human plasma [J].

Villani, P ;

Pregnolato, M ;

Banfo, S ;

Rettani, M ;

Burroni, D ;

Seminari, E ;

Maserati, R ;

Regazzi, MB .

THERAPEUTIC DRUG MONITORING, 1999, 21 (03) :346-350

← 1 →