Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry for species identification of Nonfermenting Gram-Negative Bacilli

被引：32

作者：

Almuzara, Marisa ^{[1
]}

Barberis, Claudia ^{[1
]}

Traglia, German ^{[2
]}

Famiglietti, Angela ^{[1
]}

Soledad Ramirez, Maria ^{[3
]}

Vay, Carlos ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Farm & Bioquim, Hosp Clin Jose San Martin, Lab Bacteriol,Dept Bioquim Clin, RA-1113 Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, CONICET, Fac Med, Inst Microbiol & Parasitol Med IMPaM, Buenos Aires, DF, Argentina

[3] Calif State Univ Fullerton, Dept Biol Sci, Ctr Appl Biotechnol Studies, Fullerton, CA 92634 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 112卷

关键词：

MALDI-TOF MS; Nonfermenfing Gram-Negative Bacilli; Identification; Uncommon species; CYSTIC-FIBROSIS PATIENTS; BURKHOLDERIA-CEPACIA COMPLEX; BRUKER BIOTYPER; SP-NOV; RAPID IDENTIFICATION; SEQUENCE-ANALYSIS; PANDORAEA; ACHROMOBACTER; REVEALS; PATIENT;

D O I：

10.1016/j.mimet.2015.03.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) to identify 396 Nonfermenting Gram-Negative Bacilli clinical isolates was evaluated in comparison with conventional phenotypic tests and/or molecular methods. MALDI-TOF MS identified to species level 256 isolates and to genus or complex level 112 isolates. It identified 29 genera including uncommon species. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：24 / 27

页数：4

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