Chromosomal organization and nucleotide sequence of the genes coding for the elongation factors G and Tu of the apple proliferation phytoplasma

被引：28

作者：

Berg, M ^{[1
]}

Seemüller, E ^{[1
]}

机构：

[1] Biol Bundesanstalt Land & Forstwirtschaft, Inst Pflanzenschutz Obstbau, D-69221 Dossenheim, Germany

来源：

GENE | 1999年 / 226卷 / 01期

关键词：

Mollicutes; td gene; fus gene; ribosomal protein gene; phylogeny;

D O I：

10.1016/S0378-1119(98)00552-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Genes coding for elongation factors G (Sus) and Tu (tuf) of the non-culturable apple proliferation (AP) phytoplasma were cloned and sequenced. Arrangement of these genes and identification of the ribosomal protein gene rps7 upstream of the fus gene suggest a transcriptional organization similar to that of the streptomycin operon of Escherichia coli and other bacteria. The fus and tuf genes from other tested phytoplasmas were found to be similarly linked as in the AP agent. Thus, it is likely that they show a similar chromosomal arrangement. This organization would be in contrast to that of the phylogenetically distinctly different culturable mollicutes of the genus Mycoplasma in which the tuf and fus genes are separately transcribed. (C) 1999 Elsevier Science B.V. All rights reserved.

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页码：103 / 109

页数：7

相关论文

共 28 条

[1] DETECTION OF DNA OF PLANT PATHOGENIC MYCOPLASMALIKE ORGANISMS BY A POLYMERASE CHAIN-REACTION THAT AMPLIFIES A SEQUENCE OF THE 16S RNA GENE [J].

AHRENS, U ;

SEEMULLER, E .

PHYTOPATHOLOGY, 1992, 82 (08) :828-832

[2] THE NUCLEOTIDE-SEQUENCE OF TUFB AND 4 NEARBY TRANSFER-RNA STRUCTURAL GENES OF ESCHERICHIA-COLI [J].

AN, G ;

FRIESEN, JD .

GENE, 1980, 12 (1-2) :33-39

[3]

BACHLEITNER M, 1989, FEMS MICROBIOL LETT, V57, P115, DOI 10.1016/0378-1097(89)90157-2

[4] THE SEQUENCE OF THE GENE ENCODING ELONGATION FACTOR-TU FROM CHLAMYDIA-TRACHOMATIS COMPARED WITH THOSE OF OTHER ORGANISMS [J].

COUSINEAU, B ;

CERPA, C ;

LEFEBVRE, J ;

CEDERGREN, R .

GENE, 1992, 120 (01) :33-41

[5] GTP-BINDING DOMAIN - 3 CONSENSUS SEQUENCE ELEMENTS WITH DISTINCT SPACING [J].

DEVER, TE ;

GLYNIAS, MJ ;

MERRICK, WC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (07) :1814-1818

[6]

Doyle J. J., 1987, FOCUS, V19, P11, DOI DOI 10.2307/2419362

[7] THE MINIMAL GENE COMPLEMENT OF MYCOPLASMA-GENITALIUM [J].

FRASER, CM ;

GOCAYNE, JD ;

WHITE, O ;

ADAMS, MD ;

CLAYTON, RA ;

FLEISCHMANN, RD ;

BULT, CJ ;

KERLAVAGE, AR ;

SUTTON, G ;

KELLEY, JM ;

FRITCHMAN, JL ;

WEIDMAN, JF ;

SMALL, KV ;

SANDUSKY, M ;

FUHRMANN, J ;

NGUYEN, D ;

UTTERBACK, TR ;

SAUDEK, DM ;

PHILLIPS, CA ;

MERRICK, JM ;

TOMB, JF ;

DOUGHERTY, BA ;

BOTT, KF ;

HU, PC ;

LUCIER, TS ;

PETERSON, SN ;

SMITH, HO ;

HUTCHISON, CA ;

VENTER, JC .

SCIENCE, 1995, 270 (5235) :397-403

[8] PHYLOGENY OF MYCOPLASMALIKE ORGANISMS (PHYTOPLASMAS) - A BASIS FOR THEIR CLASSIFICATION [J].

GUNDERSEN, DE ;

LEE, IM ;

REHNER, SA ;

DAVIS, RE ;

KINGSBURY, DT .

JOURNAL OF BACTERIOLOGY, 1994, 176 (17) :5244-5254

[9] Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae [J].

Himmelreich, R ;

Hilbert, H ;

Plagens, H ;

Pirkl, E ;

Li, BC ;

Herrmann, R .

NUCLEIC ACIDS RESEARCH, 1996, 24 (22) :4420-4449

[10] Phylogeny based on elongation factor Tu reflects the phenotypic features of mycoplasmas better than that based on 16S rRNA [J].

Kamla, V ;

Henrich, B ;

Hadding, U .

GENE, 1996, 171 (01) :83-87