Escherichia coli ribonuclease III:: Affinity purification of hexahistidine-tagged enzyme and assays for substrate binding and cleavage

被引：58

作者：

Amarasinghe, AK

Calin-Jageman, I

Harmouch, A

Sun, WM

Nicholson, AW

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[2] Wayne State Univ, Dept Sci Biol, Detroit, MI 48202 USA

来源：

RIBONUCLEASES, PT B | 2001年 / 342卷

关键词：

D O I：

10.1016/S0076-6879(01)42542-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：143 / 158

页数：16

共 50 条

[11]

COURT D, 1993, CONTROL MESSENGER RN, P7

[12]

DUNN JJ, 1976, J BIOL CHEM, V251, P3807

[13]

Dunn JJ, 1982, ENZYMES, P485

[14]

Fersht A., 1985, ENZYME STRUCTURE MEC

[15] Proteins binding to duplexed RNA: one motif, multiple functions [J].

Fierro-Monti, I ;

Mathews, MB .

TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (05) :241-246

[16] PURIFICATION AND PROPERTIELS OF REPLICATIVE INTERMEDIATE OF RNA BACTERIOPHAGE R17 [J].

FRANKLIN, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1966, 55 (06) :1504-&

[17] OMPT ENCODES THE ESCHERICHIA-COLI OUTER-MEMBRANE PROTEASE THAT CLEAVES T7-RNA POLYMERASE DURING PURIFICATION [J].

GRODBERG, J ;

DUNN, JJ .

JOURNAL OF BACTERIOLOGY, 1988, 170 (03) :1245-1253

[18] STRUCTURE OF SECONDARY CLEAVAGE SITES OF ESCHERICHIA-COLI RNAASEIII IN A3T RNA FROM BACTERIOPHAGE-T7 [J].

GROSS, G ;

DUNN, JJ .

NUCLEIC ACIDS RESEARCH, 1987, 15 (02) :431-442

[19] UV SHADOWING - NEW AND CONVENIENT METHOD FOR LOCATION OF ULTRAVIOLET-ABSORBING SPECIES IN POLYACRYLAMIDE GELS [J].

HASSUR, SM ;

WHITLOCK, HW .

ANALYTICAL BIOCHEMISTRY, 1974, 59 (01) :162-164

[20] Rapid mutagenesis and purification of phage RNA polymerases [J].

He, B ;

Rong, MQ ;

Lyakhov, D ;

Gartenstein, H ;

Diaz, G ;

Castagna, R ;

McAllister, WT ;

Durbin, RK .

PROTEIN EXPRESSION AND PURIFICATION, 1997, 9 (01) :142-151

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