High-performance liquid chromatographic assay with ultraviolet detection for quantification of dihydrofluorouracil in human lymphocytes:: application to measurement of dihydropyrimidine dehydrogenase activity

被引：21

作者：

Déporte-Féty, R

Picot, M

Amiand, M

Moreau, A

Campion, L

Lanoë, D

Renée, N

Milano, G

机构：

[1] Ctr Rene Gauducheau, Lab Pharmacocinet, St Herblain, France

[2] Ctr Antoine Lacassagne, Lab Oncopharmacol, F-06054 Nice, France

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 762卷 / 02期

关键词：

D O I：

10.1016/S0378-4347(01)00359-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The anticancer drug 5-fluorouracil (5FU) undergoes extensive biotransformation to 5-dihydrofluorouracil (5FUH2) by the enzyme dihydropyrimidine deshydrogenase (DPD). A new HPLC method with direct UV detection for the determination of 5FUH2 in peripheral lymphocytes has been developed to detect DPD deficiency in patients treated with 5FU-based therapy. The method has been shown to be valid over the 5FUH2 concentration range of 1.14-37.88 nmol/ml. Optimal enzymatic conditions for DPD activity measurement were studied: incubation time, protein and 5FU concentrations. The assay was successfully cross-validated with the existing method using HPLC with radiochemical detection. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：203 / 209

页数：7

共 14 条

[1] FAMILIAL DEFICIENCY OF DIHYDROPYRIMIDINE DEHYDROGENASE - BIOCHEMICAL BASIS FOR FAMILIAL PYRIMIDINEMIA AND SEVERE 5-FLUOROURACIL-INDUCED TOXICITY [J].