Transcription in the nucleus and mRNA decay in the cytoplasm are coupled processes

被引：95

作者：

Goler-Baron, Vicky ^{[1
]}

Selitrennik, Michael ^{[1
]}

Barkai, Oren ^{[1
]}

Haimovich, Gal ^{[1
]}

Lotan, Rona ^{[1
]}

Choder, Mordechai ^{[1
]}

机构：

[1] Technion Israel Inst Technol, Rappaport Fac Med, Dept Mol Microbiol, IL-31096 Haifa, Israel

来源：

GENES & DEVELOPMENT | 2008年 / 22卷 / 15期

关键词：

RNA polymerase II; mRNA decay; yeast;

D O I：

10.1101/gad.473608

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Maintaining appropriate mRNAs levels is vital for any living cell. mRNA synthesis in the nucleus by RNA polymerase II core enzyme (Pol II) and mRNA decay by cytoplasmic machineries determine these levels. Yet, little is known about possible cross-talk between these processes. The yeast Rpb4/7 is a nucleo-cytoplasmic shuttling heterodimer that interacts with Pol II and with mRNAs and is required for mRNA decay in the cytoplasm. Here we show that interaction of Rpb4/7 with mRNAs and eventual decay of these mRNAs in the cytoplasm depends on association of Rpb4/7 with Pol II in the nucleus. We propose that, following its interaction with Pol II, Rpb4/7 functions in transcription, interacts with the transcript cotranscriptionally and travels with it to the cytoplasm to stimulate mRNA decay. Hence, by recruiting Rpb4/7, Pol II governs not only transcription but also mRNA decay.

引用

页码：2022 / 2027

页数：6

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