Bone marrow-derived mesenchymal stem cells protect against experimental liver fibrosis in rats

被引：216

作者：

Zhao, Dong-Chang ^{[1
,2
,3
]}

Lei, Jun-Xia ^{[2
]}

Chen, Rui ^{[1
]}

Yu, Wei-Hua ^{[1
,2
]}

Zhang, Xiu-Ming ^{[1
,2
]}

Li, Shu-Nong ^{[1
,2
]}

Xiang, Peng ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Ctr Stem Cell Biol & Tissue Engn, Guangzhou 510080, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Dept Pathophysiol, Sch Med, Guangzhou 510080, Guangdong, Peoples R China

[3] Guangdong Prov Maternal & Childrens Hosp, Dept Pediat, Guangzhou 510010, Guangdong, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2005年 / 11卷 / 22期

基金：

中国国家自然科学基金;

关键词：

Mesenchymal stem cells; Liver fibrosis; Rat; Therapy;

D O I：

10.3748/wjg.v11.i22.3431

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: Recent reports have shown the capacity of mesenchymal stem cells (MSCs) to differentiate into hepatocytes in vitro and in vivo. MSCs administration could repair injured liver, lung, or heart through reducing inflammation, collagen deposition, and remodeling. These results provide a clue to treatment of liver fibrosis. The aim of this study was to investigate the effect of infusion of bone marrow (BM)-derived MSCs on the experimental liver fibrosis in rats. METHODS: MSCs isolated from BM in male Fischer 344 rats were infused to female Wistar rats induced with carbon tetrachloride (CCl4) or dimethylnitrosamine (DMN). There were two random groups on the 42(nd) d of CCl4: CCl4/MSCs, to infuse a dose of MSCs alone; CCl4/saline, to infuse the same volume of saline as control. There were another three random groups after exposure to DMN: DMN10/MSCs, to infuse the same dose of MSCs on d 10; DMN10/saline, to infuse the same volume of saline on d 10; DMN20/MSCs, to infuse the same dose of MSCs on d 20. The morphological and behavioral changes of rats were monitored everyday. After 4-6 wk of MSCs administration, all rats were killed and fibrosis index were assessed by histopathology and radioimmunoassay. Smooth muscle alpha-actin (alpha-SMA) of liver were tested by immunohistochemistry and quantified by IBAS 2.5 software. Male rats sex determination region on the Y chromosome (sry) gene were explored by PCR. RESULTS: Compared to controls, infusion of MSCs reduced the mortality rates of incidence in CCl4-induced model (10% vs 20%) and in DMN-induced model (20-40% vs 90%). The amount of collagen deposition and alpha-SMA staining was about 40-50% lower in liver of rats with MSCs than that of rats without MSCs. The similar results were observed in fibrosis index. And the effect of the inhibition of fibrogenesis was greater in DMN10/MSCs than in DMN20/MSCs. The sry gene was positive in the liver of rats with MSCs treatment by PCR. CONCLUSION: MSCs treatment can protect against experimental liver fibrosis in CCl4-induced or DMN-induced rats and the mechanisms of the anti-fibrosis by MSCs will be studied further. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.

引用

页码：3431 / 3440

页数：10