Molecularly imprinted polymer library on a microtiter plate. High-throughput synthesis and assessment of cinchona alkaloid-imprinted polymers

被引：22

作者：

Takeuchi, T ^{[1
]}

Seko, A ^{[1
]}

Matsui, J ^{[1
]}

Mukawa, T ^{[1
]}

机构：

[1] Hiroshima City Univ, Fac Informat Sci, Lab Synthet Biochem, Asaminami Ku, Hiroshima 7313194, Japan

来源：

INSTRUMENTATION SCIENCE & TECHNOLOGY | 2001年 / 29卷 / 01期

基金：

日本学术振兴会;

关键词：

D O I：

10.1081/CI-100001402

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A molecularly imprinted polymer library was prepared on a 96-well microtiter plate, using a liquid-handing equipment that was programmed to prepare various breeds of cinchonidine-imprinted polymers. The resultant polymers, immobilized on the bottom surface of the wells, were screened for the binding capacity by fluorescence measurement, using a microplate reader. Comparison with a conventional assessment by FIA (flow injection analysis) demonstrated the reliability and the high-throughput performance of the microtiter plate-based combinatorial molecular imprinting.

引用

页码：1 / 9

页数：9

共 15 条

[1] BARTSCH RA, 1997, ACS S SER, V703
[2] Comparison of polymer coatings of capillaries for capillary electrophoresis with respect to their applicability to molecular imprinting and electrochromatography
Bruggemann, O
Freitag, R
Whitcombe, MJ
Vulfson, EN
[J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 781 (1-2) : 43 - 53
[3] Plastic antibodies: developments and applications
Haupt, K
Mosbach, K
[J]. TRENDS IN BIOTECHNOLOGY, 1998, 16 (11) : 468 - 475
[4] Molecular recognition in cinchona alkaloid molecular imprinted polymer rods
Matsui, J
Nicholls, IA
Takeuchi, T
[J]. ANALYTICA CHIMICA ACTA, 1998, 365 (1-3) : 89 - 93
[5] ROD-TYPE AFFINITY MEDIA FOR LIQUID-CHROMATOGRAPHY PREPARED BY IN-SITU-MOLECULAR IMPRINTING
MATSUI, J
MIYOSHI, Y
MATSUI, R
TAKEUCHI, T
[J]. ANALYTICAL SCIENCES, 1995, 11 (06) : 1017 - 1019
[6] MOLECULAR RECOGNITION IN CONTINUOUS POLYMER RODS PREPARED BY A MOLECULAR IMPRINTING TECHNIQUE
MATSUI, J
KATO, T
TAKEUCHI, T
SUZUKI, M
YOKOYAMA, K
TAMIYA, E
KARUBE, I
[J]. ANALYTICAL CHEMISTRY, 1993, 65 (17) : 2223 - 2224
[7] Highly stereoselective molecularly imprinted polymer synthetic receptors for cinchona alkaloids
Matsui, J
Nicholls, IA
Takeuchi, T
[J]. TETRAHEDRON-ASYMMETRY, 1996, 7 (05) : 1357 - 1361
[8] MATSUI J, IN PRESS ANAL CHEM
[9] IMPRINTED POLYMERS AS ANTIBODY MIMETICS AND NEW AFFINITY GELS FOR SELECTIVE SEPARATIONS IN CAPILLARY ELECTROPHORESIS
NILSSON, K
LINDELL, J
NORRLOW, O
SELLERGREN, B
[J]. JOURNAL OF CHROMATOGRAPHY A, 1994, 680 (01) : 57 - 61
[10] Capillary electrochromatography with predetermined selectivity obtained through molecular imprinting
Schweitz, L
Andersson, LI
Nilsson, S
[J]. ANALYTICAL CHEMISTRY, 1997, 69 (06) : 1179 - 1183

← 1 2 →