Spatial and temporal expression of heparan sulfate in mouse development regulates FGF and FGF receptor assembly

被引:153
作者
Allen, BL [1 ]
Rapraeger, AC [1 ]
机构
[1] Univ Wisconsin, Dept Pathol & Lab Med, Program Mol & Cellular Biol, Madison, WI 53706 USA
关键词
fibroblast growth factors; FGF receptors; development; proteoglycan;
D O I
10.1083/jcb.200307053
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Heparan sulfate (HS) interacts with diverse growth factors, including Wnt, Hh, BMP, VEGF, EGF, and FGF family members, and is a necessary component for their signaling. These proteins regulate multiple cellular processes that are critical during development. However, a major question is whether developmental changes occur in HS that regulate the activity of these factors. Using a ligand and carbohydrate engagement assay, and focusing on FGF1 and FGF8b interactions with FGF receptor (FR)2c and FR3c, this paper reveals global changes in HS expression in mouse embryos during development that regulate FGF and FR complex assembly. Furthermore, distinct HS requirements are identified for both complex formation and signaling for each FGF and FR pair. Overall, these results suggest that changes in HS act as critical temporal regulators of growth factor and morphogen signaling during embryogenesis.
引用
收藏
页码:637 / 648
页数:12
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