Development of a DNA biochip: principle and applications

被引：45

作者：

Vo-Dinh, T ^{[1
]}

机构：

[1] Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1998年 / 51卷 / 1-3期

关键词：

biochip; DNA fluorescence detection; integrated circuit; human immune-deficiency virus;

D O I：

10.1016/S0925-4005(98)00182-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper provides an overview of the operating principle of a DNA biochip based on integrated circuit for use in biomedical diagnostics. The device is a self-contained system with photosensors, amplifiers, discriminators and logic circuitry on board. The development and evaluation of various microchip system components of the genosensor are reviewed. Fluorescence detection of gene probes specific to DNA sequences related to pathogens such as the human immune-deficiency virus 1 (HIV1) system illustrates the usefulness and potential of the DNA biochip technology for rapid and cost-effective medical diagnostics. Potential usefulness of the DNA biochip in clinical applications and functional genomics research is discussed. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：52 / 59

页数：8

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