Trans-splicing of a voltage-gated sodium channel is regulated by nerve growth factor

被引:68
作者
Akopian, AN
Okuse, K
Souslova, V
England, S
Ogata, N
Wood, JN
机构
[1] Univ London Univ Coll, Dept Biol, London WC1E 6BT, England
[2] Hiroshima Univ, Fac Med, Dept Physiol 2, Hiroshima 734, Japan
基金
英国惠康基金;
关键词
trans-splicing nerve growth factor; sensory neuron specific; dorsal root ganglia;
D O I
10.1016/S0014-5793(99)00126-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mammalian sensory neurons express a voltage-gated sodium channel named SNS, Here we report the identification of an SNS transcript (SNS-A) that contains an exact repeat of exons 12, 13 and 14 encoding a partial repeat of domain II. Because the exons 12-14 are present in single copies in genomic DNA, the SNS-A transcript must arise by trans-splicing. Nerve growth factor, which regulates pain thresholds, and the functional expression of voltage-gated sodium channels increases the levels of the SNS-A transcript several-fold both in vivo and in vitro as measured by RNase protection methods, as well as RT-PCR, These data demonstrate a novel regulatory role for the nerve growth factor and are the first example of trans-splicing in the vertebrate nervous system. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码:177 / 182
页数:6
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