Regulation of Pseudomonas aeruginosa hemF and hemN by the dual action of the redox response regulators Anr and Dnr

被引:72
作者
Rompf, A
Hungerer, C
Hoffmann, T
Lindenmeyer, M
Römling, U
Gross, U
Doss, MO
Arai, H
Igarashi, Y
Jahn, D
机构
[1] Albert Ludwigs Universitat Freiburg, Inst Organ Chem & Biochem, D-79104 Freiburg, Germany
[2] Univ Marburg, Fachbereich Biol, Mikrobiol Lab, D-35032 Marburg, Germany
[3] Max Planck Inst Terr Mikrobiol, D-35043 Marburg, Germany
[4] Karolinska Inst, S-17177 Stockholm, Sweden
[5] Philipps Univ Marburg, Fachbereich Human Med, Abt Klin Biochem, D-35037 Marburg, Germany
[6] Inst Phys & Chem Res, Wako, Saitama 35101, Japan
[7] Univ Tokyo, Dept Biotechnol, Bunkyo Ku, Tokyo 113, Japan
关键词
D O I
10.1046/j.1365-2958.1998.00980.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The oxidative decarboxylation of coproporphyrinogen III catalysed by an oxygen-dependent oxidase (HemF) and an oxygen-independent dehydrogenase (HemN) is one of the key regulatory points of haem biosynthesis in Pseudomonas aeruginosa, To investigate the oxygen-dependent regulation of hemF and hemN, the corresponding genes were cloned from the P. aeruginosa chromosome. Recognition sequences for the Fnr-type transcriptional regulator Anr were detected -44.5 bp from the 5' end of the hemF mRNA transcript and at an optimal distance of -41.5 bp with respect to the transcriptional start of hemN, An approximately 10-fold anaerobic induction of hemN gene expression was mediated by the dual action of Anr and a second Fnr-type regulator, Dnr, Regulation by both proteins required the Anr recognition sequence. Surprisingly, aerobic expression of hemN was dependent only on Anr, An anr mutant did not contain detectable amounts of hemN mRNA and accumulated coproporphyrin III both aerobically and anaerobically, indicating the importance of HemN for aerobic and anaerobic haem formation. Mutation of hemN and hemF did not abolish aerobic or anaerobic growth, indicating the existence of an additional HemN-type enzyme, which was termed HemZ, Expression of hemF was induced approximately 20-fold during anaerobic growth and, as was found for hemN, both Anr and Dnr were required for anaerobic induction. Paradoxically, oxygen is necessary for HemF catalysis, suggesting the existence of an additional physiological function for the P. aeruginosa HemF protein.
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页码:985 / 997
页数:13
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共 52 条
  • [41] The complete genome sequence of the gastric pathogen Helicobacter pylori
    Tomb, JF
    White, O
    Kerlavage, AR
    Clayton, RA
    Sutton, GG
    Fleischmann, RD
    Ketchum, KA
    Klenk, HP
    Gill, S
    Dougherty, BA
    Nelson, K
    Quackenbush, J
    Zhou, LX
    Kirkness, EF
    Peterson, S
    Loftus, B
    Richardson, D
    Dodson, R
    Khalak, HG
    Glodek, A
    McKenney, K
    Fitzegerald, LM
    Lee, N
    Adams, MD
    Hickey, EK
    Berg, DE
    Gocayne, JD
    Utterback, TR
    Peterson, JD
    Kelley, JM
    Cotton, MD
    Weldman, JM
    Fujii, C
    Bowman, C
    Watthey, L
    Wallin, E
    Hayes, WS
    Weidman, JM
    Fujii, C
    Borodovsky, M
    Karp, PD
    Smith, HO
    Fraser, CM
    Venter, JC
    [J]. NATURE, 1997, 388 (6642) : 539 - 547
  • [42] ISOLATION OF THE HEMF OPERON CONTAINING THE GENE FOR THE ESCHERICHIA-COLI AEROBIC COPROPORPHYRINOGEN-III OXIDASE BY IN-VIVO COMPLEMENTATION OF A YEAST HEM13 MUTANT
    TROUP, B
    JAHN, M
    HUNGERER, C
    JAHN, D
    [J]. JOURNAL OF BACTERIOLOGY, 1994, 176 (03) : 673 - 680
  • [43] CLONING AND CHARACTERIZATION OF THE ESCHERICHIA-COLI HEMN GENE ENCODING THE OXYGEN-INDEPENDENT COPROPORPHYRINOGEN-III OXIDASE
    TROUP, B
    HUNGERER, C
    JAHN, D
    [J]. JOURNAL OF BACTERIOLOGY, 1995, 177 (11) : 3326 - 3331
  • [44] Characterisation of Escherichia coli K-12 mutants defective in formate-dependent nitrite reduction: essential roles for hemN and the menFDBCE operon
    Tyson, K
    Metheringham, R
    Griffiths, L
    Cole, J
    [J]. ARCHIVES OF MICROBIOLOGY, 1997, 168 (05) : 403 - 411
  • [45] The homologous regulators ANR of Pseudomonas aeruginosa and FNR of Escherichia coli have overlapping but distinct specificities for anaerobically inducible promoters
    Winteler, HV
    Haas, D
    [J]. MICROBIOLOGY-UK, 1996, 142 : 685 - 693
  • [46] AN OXYGEN-DEPENDENT COPROPORPHYRINOGEN OXIDASE ENCODED BY THE HEMF GENE OF SALMONELLA-TYPHIMURIUM
    XU, KP
    ELLIOTT, T
    [J]. JOURNAL OF BACTERIOLOGY, 1993, 175 (16) : 4990 - 4999
  • [47] CLONING, DNA-SEQUENCE, AND COMPLEMENTATION ANALYSIS OF THE SALMONELLA-TYPHIMURIUM HEMN GENE ENCODING A PUTATIVE OXYGEN-INDEPENDENT COPROPORPHYRINOGEN-III OXIDASE
    XU, KP
    ELLIOTT, T
    [J]. JOURNAL OF BACTERIOLOGY, 1994, 176 (11) : 3196 - 3203
  • [48] THE GENES REQUIRED FOR HEME-SYNTHESIS IN SALMONELLA-TYPHIMURIUM INCLUDE THOSE ENCODING ALTERNATIVE FUNCTIONS FOR AEROBIC AND ANAEROBIC COPROPORPHYRINOGEN OXIDATION
    XU, KP
    DELLING, J
    ELLIOTT, T
    [J]. JOURNAL OF BACTERIOLOGY, 1992, 174 (12) : 3953 - 3963
  • [49] ANAEROBIC ACTIVATION OF THE ENTIRE DENITRIFICATION PATHWAY IN PSEUDOMONAS-AERUGINOSA REQUIRES ANR, AN ANALOG OF FNR
    YE, RW
    HAAS, D
    KA, JO
    KRISHNAPILLAI, V
    ZIMMERMANN, A
    BAIRD, C
    TIEDJE, JM
    [J]. JOURNAL OF BACTERIOLOGY, 1995, 177 (12) : 3606 - 3609
  • [50] ZAGOREC M, 1988, J BIOL CHEM, V263, P9718