Interaction between fast and slow inactivation in Skm1 sodium channels

被引:111
作者
Featherstone, DE
Richmond, JE
Ruben, PC
机构
[1] Department of Biology, Utah State University, Logan
关键词
D O I
10.1016/S0006-3495(96)79504-8
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Rat skeletal muscle (Skm1) sodium channel alpha and beta(1) subunits were coexpressed in Xenopus oocytes, and resulting sodium currents were recorded from on-cell macropatches. First, the kinetics and steady-state probability of both fast and slow inactivation in Skm1 wild type (WT) sodium channels were characterized. Next, we confirmed that mutation of IFM to QQQ (IFM1303QQQ) in the DIII-IV 'inactivation loop' completely removed fast inactivation at all voltages. This mutation was then used to characterize Skm1 slow inactivation without the presence of fast inactivation. The major findings of this paper are as follows: 1) Even with complete removal of fast inactivation by the IFM1303QQQ mutation, slow inactivation remains intact. 2) In WT channels, similar to 20% of channels fail to slow-inactivate after fast-inactivating, even at very positive potentials. 3) Selective removal of fast inactivation by IFM1303QQQ allows stow inactivation to occur more quickly and completely than in WT. We conclude that fast inactivation reduces the probability of subsequent slow inactivation.
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收藏
页码:3098 / 3109
页数:12
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