Houttuynia cordata blocks HSV infection through inhibition of NF-κB activation

被引:105
作者
Chen, Xiaoqing [1 ,2 ,3 ]
Wang, Zhongxia [1 ]
Yang, Ziying [2 ,3 ]
Wang, Jingjing [1 ,2 ,3 ]
Xu, Yunxia [2 ,3 ]
Tan, Ren-xiang [2 ,3 ]
Li, Erguang [1 ,3 ]
机构
[1] Nanjing Univ, Sch Med, Nanjing 210093, Jiangsu, Peoples R China
[2] Nanjing Univ, Coll Life Sci, Nanjing 210093, Peoples R China
[3] Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China
关键词
Houttuynia cordata; Herpes simplex virus; NF-kappa B; Flavonoid; Antiviral; HERPES-SIMPLEX-VIRUS; INFLUENZA-VIRUS; NUCLEAR TRANSLOCATION; TYPE-1; INFECTION; GENE-EXPRESSION; APOPTOSIS; PATHWAY; KINASE; REPLICATION; FLAVONOIDS;
D O I
10.1016/j.antiviral.2011.09.005
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Houttuynia cordata Thunb. is a medicinal plant widely used in folk medicine in several Asian countries. It has been reported that a water extract of H. cordata exhibits activity against herpes simplex virus (HSV) and the virus of severe acute respiratory syndrome (SARS), although the mechanisms are not fully understood yet. Previous studies have demonstrated absolute requirement of NF-kappa B activation for efficient replication of HSV-1 and HSV-2 and inhibition of NF-kappa B activation has been shown to suppress HSV infection. Here we show that a hot water extract of H. cordata (HCWE) inhibits HSV-2 infection through inhibition of NF-kappa B activation. The IC(50) was estimated at 50 mu g/ml of lyophilized HCWE powder. At 150 and 450 mu g/ml, HCWE blocked infectious HSV-2 production by more than 3 and 4 logs, respectively. The inhibitory activity was concomitant with an inhibition of NF-kappa B activation by HSV-2 infection. Although activation of NF-kappa B and Erk MAPK has been implicated for HSV replication and growth, HCWE showed no effect on HSV-2-induced Erk activation. Furthermore, we show that treatment with quercetin, quercitrin or isoquercitrin, major water extractable flavonoids from H. cordata, significantly blocked HSV-2 infection. These results together demonstrated that H. cordata blocks HSV-2 infection through inhibition of NF-kappa B activation. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:341 / 345
页数:5
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