Desensitization of the P2X(2) receptor controlled by alternative splicing

被引：145

作者：

Brandle, U

Spielmanns, P

Osteroth, R

Sim, J

Surprenant, A

Buell, G

Ruppersberg, JP

Plinkert, PK

Zenner, HP

Glowatzki, E

机构：

[1] UNIV TUBINGEN,DEPT OTOLARYNGOL,D-72076 TUBINGEN,GERMANY

[2] UNIV TUBINGEN,INST PHYSIOL 2,D-72076 TUBINGEN,GERMANY

[3] GLAXO WELLCOME RES & DEV SA,GENEVA BIOMED RES INST,CH-1228 GENEVA,SWITZERLAND

[4] MERZ GMBH & CO,D-60318 FRANKFURT,GERMANY

来源：

FEBS LETTERS | 1997年 / 404卷 / 2-3期

关键词：

P2X receptor; genomic sequence; alternative splicing; desensitization;

D O I：

10.1016/S0014-5793(97)00128-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

P2X receptors are ion channels gated by extracellular ATP. We report here cloning of a P2X(2) receptor splice variant (P2X(2-2)) carrying a 207 bp deletion in the intracellular C-terminus and the analysis of the corresponding genomic structure of the P2X(2) gene. P2X(2-2) is as highly expressed as the original P2X(2) sequence in various tissues. ATP-activated currents mediated by heterologous expressed P2X(2) or P2X(2-2) receptors showed significant differences in desensitization time constants and steady-state currents in the continuous presence of ATP. These results imply functional differences between cells differentially expressing these P2X(2) isoforms. (C) 1997 Federation of European Biochemical Societies.

引用

页码：294 / 298

页数：5