Molecular cloning of the cDNAs encoding two gonadotropin beta subunits (GTH-I beta and -II beta) from the goldfish, Carassius auratus

被引:102
作者
Yoshiura, Y
Kobayashi, M
Kato, Y
Aida, K
机构
[1] UNIV TOKYO,GRAD SCH AGR & LIFE SCI,DEPT AQUAT BIOSCI,BUNKYO KU,TOKYO 113,JAPAN
[2] GUNMA UNIV,BIOSIGNAL RES CTR,INST MOL & CELLULAR REGULAT,MAEBASHI,GUMMA 371,JAPAN
关键词
D O I
10.1006/gcen.1996.6839
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Two types of cDNAs (GTH-I beta and -II beta) that encode the beta subunit of goldfish gonadotropin were cloned from a goldfish pituitary cDNA library. The nucleotide sequence of GTH-I beta cDNA was 616 bp long, encoding 130 amino acids, and that of GTH-II beta was 552 bp long, encoding 140 amino acids. These two types of goldfish GTH beta subunits showed an identity of 52% in the nucleotide sequence and 38% in the amino acid sequence. When compared to mammalian GTH beta subunits, goldfish GTH-I beta showed higher homology to FSH-beta s than LH-beta s, and goldfish-II beta to LH-beta s. Genomic Southern blot analysis revealed that cyprinid species harbored genes which were homologous to goldfish GTH-I beta and -II beta, demonstrating the duality of GTH in cyprinid fish as has been shown in other teleost fish. Northern blot analysis for GTH-I beta and -II beta mRNAs in goldfish at differing stages of ovarian maturity (immature, maturing, mature, and regressed) showed that both GTH-I beta and -II beta mRNA levels increased with the progression of maturity and declined in regressed stage. These results suggested that gonadal development in the goldfish was regulated by the two GTHs. (C) 1997 Academic Press.
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页码:379 / 389
页数:11
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