Detecting single base substitutions, mismatches and bulges in DNA by temperature gradient gel electrophoresis and related methods

被引：35

作者：

Wartell, RM ^{[1
]}

Hosseini, S ^{[1
]}

Powell, S ^{[1
]}

Zhu, J ^{[1
]}

机构：

[1] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 806卷 / 01期

关键词：

reviews; temperature gradients; DNA;

D O I：

10.1016/S0021-9673(98)00149-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Temperature gradient gel electrophoresis (TGGE) and related methods can separate DNA fragments that differ by a single base pair or defect. This article describes the basic features of TGGE, and reviews the theoretical model of DNA unwinding and its ability to predict DNA mobility in a temperature gradient gel. Recent applications of TGGE and related methods that were directed at detecting point mutations, and evaluating the effects of single site defects are also reported. (C) 1998 Elsevier Science B.V.

引用

页码：169 / 185

页数：17

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